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| Status |
Public on Aug 05, 2010 |
| Title |
Young Adult reference tiling array |
| Project |
modENCODE
|
| Organism |
Caenorhabditis elegans |
| Experiment type |
Expression profiling by genome tiling array
|
| Summary |
modENCODE_submission_656
This submission comes from a modENCODE project of Robert Waterston. For full list of modENCODE projects, see http://www.genome.gov/26524648
Project Goal: Our experiments are designed to detect all C. elegans transcripts by hybridizing RNA to commerically available genome tiling arrays. To maximize the chances of detecting rare transcripts with limited expression in specific cells, we are extracting RNA from selected embryonic cells isolated by FACS and from postembryonic cells by use of the mRNA tagging method.
For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf
Keywords: Transcript tiling array analysis
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| |
|
| Overall design |
EXPERIMENT TYPE: Transcript tiling array analysis. BIOLOGICAL SOURCE: Strain: N2; Tissue: reference (YA); Developmental Stage: Young Adult 20dC 72hr post-L1; Genotype: wild type; Sex: Hermaphrodite; NUMBER OF REPLICATES: 3; EXPERIMENTAL FACTORS: Antibody EZview Red ANTI-FLAG® M2 Affinity Gel (target is FLAG); Strain N2; temperature 20; Developmental Stage Young Adult 20dC 72hr post-L1; Tissue reference (YA)
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| Web link |
http://www.ncbi.nlm.nih.gov/geo/info/ENCODE.html
http://www.modencode.org
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| |
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| Contributor(s) |
Waterston B, Miller D, Gerstein M, Wilson M, Argiwal A, Reinke V |
| Citation(s) |
21177967 |
| BioProject |
PRJNA168961 |
| |
| Submission date |
Jul 28, 2010 |
| Last update date |
May 02, 2013 |
| Contact name |
DCC modENCODE |
| E-mail(s) |
help@modencode.org
|
| Phone |
416-673-8579
|
| Organization name |
Ontario Institute for Cancer Research
|
| Lab |
modENCODE DCC
|
| Street address |
MaRS Centre, South Tower, 101 College Street, Suite 800
|
| City |
Toronto |
| State/province |
Ontario |
| ZIP/Postal code |
M5G 0A3 |
| Country |
Canada |
| |
|
| Platforms (1) |
| GPL5634 |
[Ce25b_MR] Affymetrix C. elegans Tiling 1.0R Array |
|
| Samples (3) |
| GSM571519 |
Young Adult reference (mockIP) tiling array extraction1_array1 |
| GSM571520 |
Young Adult reference (mockIP) tiling array extraction2_array1 |
| GSM571521 |
Young Adult reference (mockIP) tiling array extraction3_array1 |
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