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Series GSE232930 Query DataSets for GSE232930
Status Public on Sep 07, 2024
Title LINE1 and PRC2 control nucleolar organization and repression of the 8C-state in human ESCs [RNA-Seq_Primed_H9_L1KD]
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary To study L1 knockdown effect in mTeSR primed hES cells
 
Overall design In this study, we investigated LINE1 (L1), a major family of transposon elements (TEs) in the human genome, in primed hES cells for its roles in regulating 8-cell (8C) stage-specific totipotent genes.
mTeSR cultured primed H9 hES cells were transfected with 6-FAM (Fluorescein) labeled non-specific targeting ASO (ASO-Ctr), L1 targeting sense oligo (SO-L1) and anti-sense oligo (ASO-L1), respectively. Cells were maintained in the RSeT media post transfection. 48 hours later, FAM+ cells were FACS collected and immediately processed for RNA extraction and RNA-seq library prep.
 
Contributor(s) Zhang J, Ramalho-Santos M
Citation(s) 39413784
Submission date May 19, 2023
Last update date Dec 08, 2024
Contact name Juan Zhang
E-mail(s) tzzhangjuan@gmail.com
Organization name LTRI
Street address 25 Orde Street, Room 6-1001 MRS
City Toronto
State/province Ontario
ZIP/Postal code M5T 3H7
Country Canada
 
Platforms (1)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
Samples (6)
GSM7393675 Rep1, ASO-Ctr
GSM7393676 Rep1, sO-L1
GSM7393677 Rep1, ASO-L1
This SubSeries is part of SuperSeries:
GSE232939 LINE1 and PRC2 control nucleolar organization and repression of the 8C-state in human ESCs
Relations
BioProject PRJNA974449

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE232930_Primed_H9_hL1KD_ASO_sO-DEG_Cutoff_0.5.txt.gz 878.2 Kb (ftp)(http) TXT
GSE232930_Primed_H9_hL1KD_log2Nom_counts_cutoff_0.5_BatchCo.txt.gz 574.9 Kb (ftp)(http) TXT
GSE232930_Primed_H9_hL1KD_raw_counts_rmDup.txt.gz 528.3 Kb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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