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Series GSE233316 Query DataSets for GSE233316
Status Public on Jul 26, 2023
Title Barley seed developmental series
Organism Hordeum vulgare
Experiment type Expression profiling by high throughput sequencing
Summary To provide comprehensive spatiotemporal information about biological processes in developing grains of cultivated barley (Hordeum vulgare subsp. vulgare), we performed a transcriptomic study of the embryo, endosperm, and seed maternal tissues collected from 4 to 32 days after pollination.
 
Overall design Three main seed tissues (embryo, endosperm, and seed maternal tissues) were manually dissected at five developmental time points 4, 8, 16, 24 and 32 days after pollination (DAP). 4 DAP embryo and 32 DAP seed maternal tissues samples were omitted due to their minute size and dry nature, respectively. The total RNA was extracted, processed into the polyA-enriched libraries, and sequenced on the Illumina platform.
Web link https://doi.org/10.1093/plcell/koae095
 
Contributor(s) Kovacik M, Nowicka A, Zwyrtková J, Strejčková B, Vardanega I, Esteban E, Pasha A, Kaduchová K, Krautsova M, Červenková M, Šafář J, Provart NJ, Simon R, Pecinka A
Citation(s) 38635902
Submission date May 24, 2023
Last update date Apr 25, 2024
Contact name Ales Pecinka
E-mail(s) pecinka@ueb.cas.cz
Organization name Institute of Experimental Botany of the AS CR
Street address Šlechtitelů 31
City Olomouc
ZIP/Postal code 77900
Country Czech Republic
 
Platforms (1)
GPL22077 Illumina HiSeq 2500 (Hordeum vulgare)
Samples (39)
GSM7422421 4 DAP endosperm replicate 1 [102865_END_04_REP1]
GSM7422422 4 DAP endosperm replicate 2 [102866_END_04_REP2]
GSM7422423 4 DAP endosperm replicate 3 [102867_END_04_REP3]
Relations
BioProject PRJNA975859

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE233316_TPM_counts.xlsx 35.6 Mb (ftp)(http) XLSX
GSE233316_raw_counts.txt.gz 2.0 Mb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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