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Series GSE233461 Query DataSets for GSE233461
Status Public on Jun 17, 2024
Title The impact of conditional Opa1 Knockout on gene expression profile at single cell level during adult neurogenesis in the hippocampus
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary To investigate the function of Opa1 in the regulation of adult neurogenesis, we created a conditional Opa1-knockout mouse line by cross-breeding Nestin-CreERT2;ROSA26YFP mice with Opa1-Flox mice.
Overall design Using Tamoxifen, we induced Opa1 knockout on 8-weeks-old Nestin-CreERT2;ROSA26YFP;Opa1-Flox mice. We then sorted YFP-positive cells from the hippocampus using flow cytometry at 12 weeks. Single cell transcriptome was performed on the 10XGenomics platform.
Contributor(s) Slack R, Iqbal MA
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Submission date May 25, 2023
Last update date Jun 18, 2024
Contact name Ruth Slack
Phone 6135625800
Organization name University of Ottawa
Department Cellular and Molecular Medicine
Street address 451 Smyth Road
City Ottawa
State/province Ontario
ZIP/Postal code K1H 8M5
Country Canada
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (2)
GSM7428953 WT, scRNAseq
GSM7428954 Opa1cKO, scRNAseq
This SubSeries is part of SuperSeries:
GSE233465 The impact of conditional Opa1 Knockout
BioProject PRJNA976307

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Supplementary file Size Download File type/resource
GSE233461_RAW.tar 52.4 Mb (http)(custom) TAR (of MTX, TSV)
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Raw data are available in SRA
Processed data provided as supplementary file

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