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| Status |
Public on Jun 17, 2024 |
| Title |
The impact of conditional Opa1 Knockout on gene expression profile at single cell level during adult neurogenesis in the hippocampus |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
To investigate the function of Opa1 in the regulation of adult neurogenesis, we created a conditional Opa1-knockout mouse line by cross-breeding Nestin-CreERT2;ROSA26YFP mice with Opa1-Flox mice.
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| Overall design |
Using Tamoxifen, we induced Opa1 knockout on 8-weeks-old Nestin-CreERT2;ROSA26YFP;Opa1-Flox mice. We then sorted YFP-positive cells from the hippocampus using flow cytometry at 12 weeks. Single cell transcriptome was performed on the 10XGenomics platform.
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| Contributor(s) |
Slack R, Iqbal MA |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
May 25, 2023 |
| Last update date |
Jun 18, 2024 |
| Contact name |
Ruth Slack |
| E-mail(s) |
bingliusong@gmail.com
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| Phone |
6135625800
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| Organization name |
University of Ottawa
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| Department |
Cellular and Molecular Medicine
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| Street address |
451 Smyth Road
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| City |
Ottawa |
| State/province |
Ontario |
| ZIP/Postal code |
K1H 8M5 |
| Country |
Canada |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (2) |
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| This SubSeries is part of SuperSeries: |
| GSE233465 |
The impact of conditional Opa1 Knockout |
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| Relations |
| BioProject |
PRJNA976307 |
