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| Status |
Public on Jul 25, 2023 |
| Title |
The sex-specific factor SOA establishes X chromosome dosage compensation in Anopheles mosquitos [SOAR-RNA-seq] |
| Organism |
Anopheles gambiae |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
The Anopheles mosquito is one of thousands of species in which sex differences play a central role in their biology, as only females need a blood meal in order to produce eggs. Sex differentiation is regulated by sex chromosomes, but their presence creates a dosage imbalance between males (XY) and females (XX). Dosage compensation (DC) can re-equilibrate the expression of sex-chromosomal genes, but because DC mechanisms have only been fully characterized in a few model organisms, key questions about its evolutionary diversity and functional necessity remain unresolved. Here we report the discovery of a previously uncharacterized gene (SOA) as a master regulator of DC in the malaria mosquito Anopheles gambiae. Sex-specific alternative splicing prevents functional SOA protein expression in females. The male isoform encodes a DNA-binding protein that binds the promoters of active X chromosomal genes. Expressing male SOA is sufficient to induce DC in female cells. Male mosquitoes lacking SOA or female mosquitos ectopically expressing the male isoform exhibit X chromosome misregulation, which is compatible with viability but causes developmental delay. Thus, our molecular analysis of the first DC master regulator in a non-model organism elucidates the evolutionary steps leading to the establishment of a chromosome-specific fine-tuning mechanism.
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| Overall design |
Comparative gene expression profiling analysis of RNA-seq data for female pupae of wild-type and SOA-R knock-in genotype
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| Contributor(s) |
Kalita AI, Marois E, Basilicata MF, Kozielska M, Weissing FJ, Jaouen E, Möckel M, Rühle F, Butter F, Keller Valsecchi CI |
| Citation(s) |
37769784 |
| |
| Submission date |
May 25, 2023 |
| Last update date |
Oct 24, 2023 |
| Contact name |
Frank Rühle |
| E-mail(s) |
f.ruehle@imb-mainz.de
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| Organization name |
Institute of Molecular Biology (IMB)
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| Department |
Bioinformatics Core Facility
|
| Street address |
Ackermannweg 4
|
| City |
Mainz |
| ZIP/Postal code |
55128 |
| Country |
Germany |
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| Platforms (1) |
| GPL22033 |
Illumina NextSeq 500 (Anopheles gambiae) |
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| Samples (7)
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| This SubSeries is part of SuperSeries: |
| GSE210630 |
SOA confers X chromosome dosage compensation in Anopheles gambiae mosquitoes |
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| Relations |
| BioProject |
PRJNA976314 |
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