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| Status |
Public on Mar 27, 2024 |
| Title |
Barcode lineage tracking of ferret cortical progeny using TrackerSeq (Mayer lab) |
| Organism |
Mustela putorius furo |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Diversity of cortical radial glia cells (RGCs) and their complex relationships to generate neurons in species with expanded germinal zones and a folded cortex, remains unclear. We used TrackerSeq, a technique that integrates DNA barcodes into the genome of electroporated RGCs, to identify the distinct cell lineages that shape ferret cortex.
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| Overall design |
E34 ferret embryos were electroporated with a piggyBac transposon-based library and eGFP+ cells were isolated at E37 by FACS and procesed for their parallel transcriptomic and barcode-lineage tracing characterization.
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| Web link |
https://doi.org/10.1126/sciadv.adn9998
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| Contributor(s) |
Del-Valle-Anton L, Amin S, Cimino D, Neuhaus F, Dvoretskova E, Fernández V, Babal YK, Garcia-Frigola C, Prieto-Colomina A, Murcia-Ramón R, Nomura Y, Cárdenas A, Feng C, Moreno-Bravo J, Götz M, Mayer C, Borrell V |
| Citation(s) |
38536915 |
| Submission date |
May 26, 2023 |
| Last update date |
Apr 04, 2024 |
| Contact name |
Lucia Del-Valle-Anton |
| Organization name |
Instituto de Neurociencias, Consejo Superior de Investigaciones Científicas & Universidad Miguel Hernández
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| Lab |
Borrell Lab
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| Street address |
Av. Santiago Ramón y Cajal s/n
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| City |
Sant Joan d’Alacant |
| ZIP/Postal code |
03550 |
| Country |
Spain |
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| Platforms (1) |
| GPL28629 |
Illumina NovaSeq 6000 (Mustela putorius furo) |
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| Samples (8)
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| This SubSeries is part of SuperSeries: |
| GSE234305 |
Multiple parallel cell lineages in the developing mammalian cerebral cortex |
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| Relations |
| BioProject |
PRJNA976704 |
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