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| Status |
Public on Apr 12, 2024 |
| Title |
Ketogenic diet induces p53-dependent cell senescence in multiple organs |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Mice on two different ketogenic diets induce p53 and cellular senescence in multiple organs, including heart and kidney. This is mediated through inactivation of MDM2 by caspase-2 cleavage, leading to p53 accumulation and induction of p21. Ketogenic diet also induced pAMPK, suggesting that persistent activation leads to p53-dependent senescence.
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| Overall design |
Mice were placed on ketogenic diet for three weeks. Their tissues (heart, kidney and liver) were collected (3 replicates each) and analyzed via immunoblotting and RNA-seq as compared to mice on a standard chow diet.
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| Contributor(s) |
Horikoshi N, Gius D, Varmazyad M, Schell JR, Wei S, Munkacsy EE, Chen Y, Lai Z |
| Citation(s) |
38758782 |
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| Submission date |
May 31, 2023 |
| Last update date |
May 31, 2024 |
| Contact name |
Yidong Chen |
| E-mail(s) |
cheny8@uthscsa.edu
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| Phone |
2105629163
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| Organization name |
UT Health Science Center at San Antonio
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| Department |
Population Health Sciences
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| Street address |
8403 Floyd Curl Drive, MSC 7784
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| City |
San Antonio |
| State/province |
Texas |
| ZIP/Postal code |
78229 |
| Country |
USA |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (18)
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| GSM7437608 |
WT kidney, 21 days keto, biol rep 1 |
| GSM7437609 |
WT kidney, 21 days keto, biol rep 2 |
| GSM7437610 |
WT kidney, 21 days keto, biol rep 3 |
| GSM7437611 |
WT liver, 21 days keto, biol rep 1 |
| GSM7437612 |
WT liver, 21 days keto, biol rep 2 |
| GSM7437613 |
WT liver, 21 days keto, biol rep 3 |
| GSM7437614 |
WT heart, normal diet, biol rep 1 |
| GSM7437615 |
WT heart, normal diet, biol rep 2 |
| GSM7437616 |
WT heart, normal diet, biol rep 3 |
| GSM7437617 |
WT kidney, normal diet, biol rep 1 |
| GSM7437618 |
WT kidney, normal diet, biol rep 2 |
| GSM7437619 |
WT kidney, normal diet, biol rep 3 |
| GSM7437620 |
WT liver, normal diet, biol rep 1 |
| GSM7437621 |
WT liver, normal diet, biol rep 2 |
| GSM7437622 |
WT liver, normal diet, biol rep 3 |
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| Relations |
| BioProject |
PRJNA978062 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE233831_RAW.tar |
33.4 Mb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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