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Series GSE23401 Query DataSets for GSE23401
Status Public on Aug 04, 2010
Title Streptomyces coelicolor M600 BldD ChIP-chip comparison of wild-type with bldD null mutant
Organism Streptomyces coelicolor
Experiment type Genome binding/occupancy profiling by array
Summary BldD is a transcriptional regulator essential for morphological development and antibiotic production in Streptomyces coelicolor. Here we identify the BldD regulon by means of chromatin immunoprecipitation. The BldD regulon encompasses ~167 transcriptional units, of which more than 20 are known to play important roles in development and/or secondary metabolism. Strikingly, 42 BldD target genes (~25% of the regulon) encode regulatory proteins, stressing the central, pleiotropic role of BldD. Almost all BldD-binding sites identified by ChIP-chip are present in the promoters of the target genes. An exception is the tRNA gene bldA, where BldD binds within the region encoding the primary transcript, immediately downstream of the position corresponding to the processed, mature 3′-end of the tRNA. We identified a novel BldD target gene (cdgA) that influences differentiation and antibiotic production. cdgA encodes a GGDEF domain protein, implicating c-di-GMP in the regulation of Streptomyces development.
 
Overall design ChIP-chip exp comparing IP with BldD antibody against total DNA control in wild-type strain vs bldD mutant strain. Wild-type strain was analysed in triplicate
 
Contributor(s) den Hengst C, Tran N, Bibb M, Chandra G, Leskiw B, Buttner M
Citation(s) 20979333
Submission date Aug 03, 2010
Last update date Jan 16, 2024
Contact name Chris den Hengst
E-mail(s) chris.den-hengst@bbsrc.ac.uk
Phone +44 (0) 01603 450796
Fax +44 (0) 01603 450778
URL http://intranet/infoserv/cgi-bin/directory/default.asp?k=&q=buttner
Organization name John Innes Centre
Department Molecular Microbiology
Lab Mark Buttner
Street address Colney
City Norwich
State/province Norfolk
ZIP/Postal code NR4 7UH
Country United Kingdom
 
Platforms (1)
GPL10757 Oxford Gene Technology Streptomyces coelicolor 4x44K format
Samples (4)
GSM574054 wild type bldd1
GSM574055 wild type bldd2
GSM574056 wild type bldd3
Relations
BioProject PRJNA131303

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE23401_RAW.tar 35.5 Mb (http)(custom) TAR (of TXT)
GSE23401_processed_data.txt.gz 464.2 Kb (ftp)(http) TXT
Processed data included within Sample table
Processed data are available on Series record

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