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| Status |
Public on Feb 27, 2024 |
| Title |
Single-cell RNA-sequencing of stromal vascular cells (SVCs) from the epididymal fat of obese mice. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The obesity epidemic continues to worsen worldwide, driving metabolic and chronic inflammatory diseases. Thiazolidinediones (TZDs), such as rosiglitazone (Rosi), are PPARy ligands that promote anti-inflammatory changes in adipose tissue macrophages (ATMs) and cause insulin sensitization. We performed single-cell RNA-seuqnecing (scRNA-seq) to analyze the diversity and the phenotype of ATM subpopulations under Rosi and ATM-Exosome treatment.
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| Overall design |
We performed a single-cell transcriptome analysis of stromal vascular cells of the epididymal fat isolated from obese mice treated for the last month with Rosiglitazone or with ATM-Exosomes or PBS Ctrl liposomes. Obese mice were fed with HFD for 10 weeks and were then treated for 4 weeks with Rosi-ATM-Exosomes (Rosi-ATM-Exos, n=3), HFD-ATM-Exos (n=4), or PBS Ctrl liposomes (n=3) (10^9 Exos twice a week) compared to Rosi diet (HFD containing 3 mg/kg/day Rosiglitazone, n=3).
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| Web link |
https://pubmed.ncbi.nlm.nih.gov/38605183/
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| Contributor(s) |
Rohm TV, Olefsky JM |
| Citation(s) |
38605183 |
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| Submission date |
Jun 02, 2023 |
| Last update date |
May 28, 2024 |
| Contact name |
Theresa Rohm |
| E-mail(s) |
trohm@health.ucsd.edu
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| Organization name |
UCSD
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| Street address |
9500 Gilman Dr
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| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92093 |
| Country |
USA |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (13)
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| GSM7441715 |
A1, PBS.Lipo, scRNAseq |
| GSM7441716 |
A2, PBS.Lipo, scRNAseq |
| GSM7441717 |
C8, ROSI.ATM.exos, scRNAseq |
| GSM7441718 |
B5, HFD.ATM.exos, scRNAseq |
| GSM7441719 |
D11, HFD.ROSI.diet, scRNAseq |
| GSM7441720 |
A3, PBS.Lipo, scRNAseq |
| GSM7441721 |
C9, ROSI.ATM.exos, scRNAseq |
| GSM7441722 |
B6, HFD.ATM.exos, scRNAseq |
| GSM7441723 |
D12, HFD.ROSI.diet, scRNAseq |
| GSM7441724 |
B13, HFD.ATM.exos, scRNAseq |
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| Relations |
| BioProject |
PRJNA979233 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE234046_RAW.tar |
1.2 Gb |
(http)(custom) |
TAR (of H5) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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