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| Status |
Public on Jun 09, 2023 |
| Title |
Cas9-Mediated Knockout of Ndrg2 Enhances the Regenerative Potential of Dendritic Cells for Wound Healing |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We performed single-cell RNA sequencing on bone marrow derived, cultured dendritic cells after CRISPR-knockout of Ndrg2, after vitamin D3 treatment or no treatment. In addition we performed single-cell RNA sequencing of wounds that had been treated with hydrogels seeded with Ndrg2-KO dendritic cells, control (untreated) dendritic cells or blank hydrogels.
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| Overall design |
scRNA-seq was performed on murine cells collected from parabiome excisional wounds. Cells labeled as GFP+ were isolated and sequenced to analyze cells in the circulation that hone to the wound upon treatment.
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| Contributor(s) |
Henn D, Zhao D, Sivaraj D, Chen K, Qi S, Gurtner G |
| Citation(s) |
37550295 |
| |
| Submission date |
Jun 05, 2023 |
| Last update date |
Sep 07, 2023 |
| Contact name |
Kellen Chen |
| E-mail(s) |
chen.kellen@gmail.com
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| Organization name |
University of Arizona
|
| Department |
Surgery
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| Street address |
1501 N Campbell Ave
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| City |
Tucson |
| State/province |
AZ |
| ZIP/Postal code |
85724 |
| Country |
USA |
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| Platforms (1) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA980198 |
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