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Series GSE234145 Query DataSets for GSE234145
Status Public on Jun 09, 2023
Title Cas9-Mediated Knockout of Ndrg2 Enhances the Regenerative Potential of Dendritic Cells for Wound Healing
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary We performed single-cell RNA sequencing on bone marrow derived, cultured dendritic cells after CRISPR-knockout of Ndrg2, after vitamin D3 treatment or no treatment. In addition we performed single-cell RNA sequencing of wounds that had been treated with hydrogels seeded with Ndrg2-KO dendritic cells, control (untreated) dendritic cells or blank hydrogels.
Overall design scRNA-seq was performed on murine cells collected from parabiome excisional wounds. Cells labeled as GFP+ were isolated and sequenced to analyze cells in the circulation that hone to the wound upon treatment.
Contributor(s) Henn D, Zhao D, Sivaraj D, Chen K, Qi S, Gurtner G
Citation(s) 37550295
Submission date Jun 05, 2023
Last update date Sep 07, 2023
Contact name Kellen Chen
Organization name University of Arizona
Department Surgery
Street address 1501 N Campbell Ave
City Tucson
State/province AZ
ZIP/Postal code 85724
Country USA
Platforms (1)
GPL21103 Illumina HiSeq 4000 (Mus musculus)
Samples (6)
GSM7447504 DH011
GSM7447505 DH012VitD
BioProject PRJNA980198

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Supplementary file Size Download File type/resource
GSE234145_RAW.tar 383.8 Mb (http)(custom) TAR (of MTX, TSV)
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Raw data are available in SRA
Processed data provided as supplementary file

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