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| Status |
Public on Jun 01, 2024 |
| Title |
NF-κB-induced expression of T-bet suppresses proliferation of malignant B cells and is associated with good prognosis in chronic lymphocytic leukemia [scATAC-seq] |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
The T-box transcription factor T-bet is encoded by Tbx21 and was identified to play a role in aging and tissue localization of B cells. T-bet expression was detected in malignant B cells but its potential role in B-cell malignancies remains largely unexplored. We used single cell RNA sequencing (scRNA-seq) and the assay for transposase accessible chromatin using sequencing (scATAC-seq) to analyze the regulatory role of T-bet in TCL1 leukemia.
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| Overall design |
Tbx21-KO (-/-) and Tbx21-WT (+/+) TCL1 cells were expanded in immunodeficient NSG animals and analyzed using scRNA-seq and scATAC-seq.
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| Contributor(s) |
Seufert I, Rippe K, Roessner P, Seiffert M |
| Citation(s) |
38684038 |
| |
| Submission date |
Jun 06, 2023 |
| Last update date |
Jun 25, 2024 |
| Contact name |
Isabelle Seufert |
| Organization name |
DKFZ
|
| Street address |
Im Neuenheimer Feld 280
|
| City |
Heidelberg |
| ZIP/Postal code |
69120 |
| Country |
Germany |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (4)
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| GSM7457615 |
TCL1, Tbx21-WT, replicate 1, scATAC-seq |
| GSM7457616 |
TCL1, Tbx21-WT, replicate 2, scATAC-seq |
| GSM7457617 |
TCL1, Tbx21-KO, replicate 1, scATAC-seq |
| GSM7457618 |
TCL1, Tbx21-KO, replicate 2, scATAC-seq |
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| This SubSeries is part of SuperSeries: |
| GSE234226 |
NF-κB-induced expression of T-bet suppresses proliferation of malignant B cells and is associated with good prognosis in chronic lymphocytic leukemia |
|
| Relations |
| BioProject |
PRJNA980577 |
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