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Series GSE234229 Query DataSets for GSE234229
Status Public on Jan 01, 2024
Title Double-stranded RNA triggers a distinct integrated stress response in the early embryo [dsRNA tp53]
Organism Danio rerio
Experiment type Expression profiling by high throughput sequencing
Summary Gene functional study and the emerging mRNA therapy technology heavily rely on in vitro transcribed (IVT) mRNA, but the double-stranded RNA (dsRNA) by-product generated during IVT is often recognized by cells as a marker of virus replication, leading to the integrated stress response (ISR).The biological effects of dsRNA in undifferentiated cells differ significantly from those of differentiated counterparts.Here, we found that dsRNA triggered an unprecedented ISR in zebrafish early embryos, leading to developmental delay, severe cell necrosis, and lethal malformations.Mechanistically, dsRNA stimulation caused a PKR-eIF2α independent global translation inhibition and a burst of p53 signaling. In turn, p53 activated Interferon Stimulated Genes (ISGs) and other defense-related genes in the absence of the IFN system. Unexpectedly, p53 does not promote cell death; instead, it plays a protective role in weakening the damage of translation repression by compromising protein turnover via enhanced ISGylation.This study thus unveils a distinct ISR upon dsRNA stimulation, which may help perform faithful gene overexpression in early embryos and shed light on the strategies of undifferentiated cells against RNA virus infection.
 
Overall design To investigate the impact of the dsRNA-induced stress response, we compared the transcriptomes between uninjected and dsRNA-injected zebrafish MZtp53 embryos at 6 hpf
Comparative gene expression profiling analysis of RNA-seq data for MZtp53 uninjected embryos and its injected dsRNA embryos
 
Contributor(s) Lu T, Shao M
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date Jun 06, 2023
Last update date Jan 01, 2024
Contact name tong lu
E-mail(s) 202020327@mail.sdu.edu.cn
Organization name ShanDong University
Street address 72, binghai rd
City Qingdao
ZIP/Postal code 266207
Country China
 
Platforms (1)
GPL14875 Illumina HiSeq 2000 (Danio rerio)
Samples (12)
GSM7457645 wt-uninj-1
GSM7457646 wt-uninj-2
GSM7457647 wt-uninj-3
This SubSeries is part of SuperSeries:
GSE234230 Double-stranded RNA triggers a distinct integrated stress response in the early embryo
Relations
BioProject PRJNA980587

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE234229_MZtp53-VS-MZtp53_dsRNA_differential_expression_all_genes.txt.gz 4.6 Mb (ftp)(http) TXT
GSE234229_MZtp53-VS-MZtp53_dsRNA_differential_expression_genes.txt.gz 280.8 Kb (ftp)(http) TXT
GSE234229_WT-VS-WT_dsRNA_differential_expression_all_genes.txt.gz 4.6 Mb (ftp)(http) TXT
GSE234229_WT-VS-WT_dsRNA_significantly_differential_expressed_genes.txt.gz 278.7 Kb (ftp)(http) TXT
GSE234229_WT-VS_tp53_differential_expression_all_genes.txt.gz 4.6 Mb (ftp)(http) TXT
GSE234229_WT-VS_tp53_significantly_differential_expressed_genes.txt.gz 763.5 Kb (ftp)(http) TXT
GSE234229_WT_dsRNA-VS-MZtp53_dsRNA_differential_expression_all_genes.txt.gz 4.6 Mb (ftp)(http) TXT
GSE234229_WT_dsRNA-VS-MZtp53_dsRNA_differential_expression_genes.txt.gz 795.3 Kb (ftp)(http) TXT
GSE234229_all_genes_expression.fpkm_anno.txt.gz 4.4 Mb (ftp)(http) TXT
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