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| Status |
Public on Jun 27, 2023 |
| Title |
Expression data of Tubb3+ cells from E4 chicken neural tube |
| Organism |
Gallus gallus |
| Experiment type |
Expression profiling by array
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| Summary |
The specification of the different neuronal subtypes is associated with a profound morphological transformation, involving processes common to most neurons and others characteristic of each neuronal subtype. To study the Tcf-dependent genes that were active during commissural neuron differentiation, we compared the genes in differentiating neurons (Tubb3+ cells) after the repression of Tcf-dependet transcription in chichen neural tube from E3 to E4, period when mostly commissural neurons are differentiating.
We used Affymetrix GeneChip arrays to study TCF regulated genes during this process.
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| Overall design |
We transfected E3 chick embryos for 24 h to produce either Tubb3::CAG:TcfEnR (suppression of Tcf-dependent transcription in neurons) or Tubb3::CAG:control (controls), dissected the NTs and cell-sorted the GFP+ neural populations. Both vectors are bicistronic and contain GFP CDS after IRES.
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| Contributor(s) |
Herrera A, Pons S |
| Citation(s) |
37519286 |
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| Submission date |
Jun 08, 2023 |
| Last update date |
Sep 15, 2023 |
| Contact name |
Antonio Herrera |
| E-mail(s) |
toniherrerac@gmail.com
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| Organization name |
IBMB-CSIC
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| Street address |
Baldiri i Reixac
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| City |
Barcelona |
| ZIP/Postal code |
08028 |
| Country |
Spain |
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| Platforms (1) |
| GPL3213 |
[Chicken] Affymetrix Chicken Genome Array |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA981570 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE234518_RAW.tar |
24.7 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data included within Sample table |
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