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Series GSE234543 Query DataSets for GSE234543
Status Public on Apr 12, 2024
Title Long Non-Coding RNA MEG3 Promotes Angiogenesis by directly regulating integrin signaling impairing endothelial cell function [ChIP-seq]
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary We immunoprecipitated EZH2, together with associated chromatin isolated from the HUVECs (2 x 10^6) that were transfected with MEG3 GapmeRs (10 nM, 48 h) or a scrambled control GapmeRs (Ctr). For transfection, we used 10 nM scrambled LNA (locked nucleic acids) GapmeR control (Cat. No. 339515) or phosphorothioate antisense standard GapmeRs MEG3-lncRNA (Cat No. 339511, Qiagen). On beads crosslinked chromatin complexes were reversed, and DNA purified using QIAquick® PCR Purification Kit and quantified by Qubit HS assay (Q33230)
Overall design Eight different ChIRP experiments were carried out using human HUVEC cells, with MEG3 or LacZ control biotynilated probes used as bait against MEG3-lncRNA.
Contributor(s) Dudnakova T, Dunn-Davies H, Nogara A, Rodor J, Thomas A, Parish E, Gautier P, Meynert A, Madeddu P, Caporali A, Baker A, Tollervey D, Mitic T
Citation(s) 38617973
Submission date Jun 09, 2023
Last update date May 01, 2024
Contact name Hywel Dunn-Davies
Organization name University of Edinburgh
Department Wellcome Trust Centre for Cell Biology
Lab Tollervey Lab
Street address Max Born Crescent
City Edinburgh
ZIP/Postal code EH9 3BF
Country United Kingdom
Platforms (1)
GPL20301 Illumina HiSeq 4000 (Homo sapiens)
Samples (6)
GSM7470574 Scr control EZH2 replicate 1, ChIPseq
GSM7470575 Scr control EZH2 replicate 2, ChIPseq
GSM7470576 Scr control EZH2 replicate 3, ChIPseq
This SubSeries is part of SuperSeries:
GSE234544 Long Non-Coding RNA MEG3 Promotes Angiogenesis by directly regulating integrin signaling impairing endothelial cell function.
BioProject PRJNA982001

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Supplementary file Size Download File type/resource
GSE234543_RAW.tar 210.0 Kb (http)(custom) TAR (of BED)
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Raw data are available in SRA
Processed data provided as supplementary file

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