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| Status |
Public on Apr 12, 2024 |
| Title |
Long Non-Coding RNA MEG3 Promotes Angiogenesis by directly regulating integrin signaling impairing endothelial cell function [ChIP-seq] |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
We immunoprecipitated EZH2, together with associated chromatin isolated from the HUVECs (2 x 10^6) that were transfected with MEG3 GapmeRs (10 nM, 48 h) or a scrambled control GapmeRs (Ctr). For transfection, we used 10 nM scrambled LNA (locked nucleic acids) GapmeR control (Cat. No. 339515) or phosphorothioate antisense standard GapmeRs MEG3-lncRNA (Cat No. 339511, Qiagen). On beads crosslinked chromatin complexes were reversed, and DNA purified using QIAquick® PCR Purification Kit and quantified by Qubit HS assay (Q33230)
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| Overall design |
Eight different ChIRP experiments were carried out using human HUVEC cells, with MEG3 or LacZ control biotynilated probes used as bait against MEG3-lncRNA.
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| Contributor(s) |
Dudnakova T, Dunn-Davies H, Nogara A, Rodor J, Thomas A, Parish E, Gautier P, Meynert A, Madeddu P, Caporali A, Baker A, Tollervey D, Mitic T |
| Citation(s) |
38617973 |
| |
| Submission date |
Jun 09, 2023 |
| Last update date |
May 01, 2024 |
| Contact name |
Hywel Dunn-Davies |
| Organization name |
University of Edinburgh
|
| Department |
Wellcome Trust Centre for Cell Biology
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| Lab |
Tollervey Lab
|
| Street address |
Max Born Crescent
|
| City |
Edinburgh |
| ZIP/Postal code |
EH9 3BF |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL20301 |
Illumina HiSeq 4000 (Homo sapiens) |
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| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE234544 |
Long Non-Coding RNA MEG3 Promotes Angiogenesis by directly regulating integrin signaling impairing endothelial cell function. |
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| Relations |
| BioProject |
PRJNA982001 |
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