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| Status |
Public on Jul 03, 2023 |
| Title |
Characterizing control of memory CD8 T cell differentiation by BTB-ZF transcription factor Zbtb20 using single cell RNA-sequencing (scATAC-Seq) |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Members of the BTB-ZF transcription factor family regulate the immune system. Our lab identified that family member Zbtb20 contributes to the differentiation, recall responses and metabolism of CD8 T cells. Here, we report a characterization of the transcriptional and epigenetic signatures controlled by Zbtb20 at single-cell resolution during the effector and memory phases of the CD8 T cell response. Without Zbtb20, transcriptional programs associated with memory CD8 T cell formation were upregulated throughout the CD8 T response. A signature of open chromatin was associated with genes controlling T cell activation, consistent with the known impact on differentiation. Additionally, memory CD8 T cells lacking Zbtb20 were characterized by open chromatin regions with overrepresentation of AP-1 transcription factor motifs and elevated RNA- and protein-level expression of the corresponding AP-1 components. Finally, we describe motifs and genomic annotations from the DNA targets of Zbtb20 in CD8 T cells identified by CUT&RUN. Together, these data establish the transcriptional and epigenetic networks contributing to the control of CD8 T cell responses by Zbtb20.
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| Overall design |
4 samples of murine OT-I CD8 T cells responding to Listeria-actA-OVA infection. Each sample is the result of pooling OT-I cells from the splenocytes of four individual mice. Of these samples, 2 were collected 10 days post-infection and 2 were collected 35 days post-infection. At each time point, 1 sample is WT OT-I cells and 1 sample is Zbtb20 KO OT-I cells.
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| Contributor(s) |
Preiss NK, Wilkins OM, Usherwood EJ |
| Citation(s) |
37414528 |
| |
| Submission date |
Jun 09, 2023 |
| Last update date |
Sep 11, 2023 |
| Contact name |
Edward Usherwood |
| E-mail(s) |
d1261h4@dartmouth.edu
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| Phone |
16036465224
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| Organization name |
Geisel School of Medicine at Dartmouth College
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| Department |
Microbiology and Immunology
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| Street address |
Rubin Building, Level 7, 710-53
|
| City |
Lebanon |
| State/province |
NH |
| ZIP/Postal code |
03756 |
| Country |
USA |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (4)
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| This SubSeries is part of SuperSeries: |
| GSE234576 |
Characterizing control of memory CD8 T cell differentiation by BTB-ZF transcription factor Zbtb20 using single cell RNA-sequencing |
|
| Relations |
| BioProject |
PRJNA982077 |
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