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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Apr 17, 2024 |
| Title |
Effects of selective elimination of senescent cells on RPE/choroid complex in mice with subretinal drusenoid deposits |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The target deletion of ATP binding cassette subfamily member 1 (ABCA1) and G1 (ABCG1) in myeloid cells leads to subretinal drusenoid deposits in mice (Abca1/g1 -m/-m). We here report the selective elimination of p16-positive cells results in the significant eduction in myeloid markers, pro-inflammatory cytokines and prostaglandin biosynthetic enzymes.
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| Overall design |
INK-ATTAC mice are transgenic mice expressing FKBP-caspase 8 fusion protein under INK4a promoter. p16Ink4a-positive senescent cells can be effectively ablated by the administration of AP20187 which dimerizes the fusion protein and selectively induce apoptosis of p16-positive senescent cells.To ablate p16Ink4a-positive cells, Abca1/g1 -m/-m; INK-ATTAC mice were treated with intraperitoneal injection of AP20187 every other day for 3 months. RPE/choroid complex samples were collected from eyes.
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| Contributor(s) |
Apte RS, Yoshida M |
| Citation(s) |
38636518 |
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| Submission date |
Jun 09, 2023 |
| Last update date |
Jul 18, 2024 |
| Contact name |
Ryo Terao |
| E-mail(s) |
terao@wustl.edu
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| Phone |
314-362-6690
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| Organization name |
Washington University School of Medicine in St. Louis
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| Department |
Ophthalmology & Visual Sciences
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| Street address |
517 S. Euclid Ave
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| City |
St. Louis |
| State/province |
MO |
| ZIP/Postal code |
63110 |
| Country |
USA |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (15)
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| GSM7472508 |
RPE/choroid complex, control 4 |
| GSM7472509 |
RPE/choroid complex, control 5 |
| GSM7472510 |
RPE/choroid complex, control 6 |
| GSM7472511 |
RPE/choroid complex, control 7 |
| GSM7472512 |
RPE/choroid complex, INK-ATTAC 1 |
| GSM7472513 |
RPE/choroid complex, INK-ATTAC 2 |
| GSM7472514 |
RPE/choroid complex, INK-ATTAC 3 |
| GSM7472515 |
RPE/choroid complex, INK-ATTAC 4 |
| GSM7472516 |
RPE/choroid complex, INK-ATTAC 5 |
| GSM7472517 |
RPE/choroid complex, INK-ATTAC 6 |
| GSM7472518 |
RPE/choroid complex, INK-ATTAC 7 |
| GSM7472519 |
RPE/choroid complex, INK-ATTAC 8 |
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| Relations |
| BioProject |
PRJNA982173 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE234641_all.gene_counts.tsv.gz |
1.6 Mb |
(ftp)(http) |
TSV |
| GSE234641_group_ink_l_Differential_Expression.tsv.gz |
2.4 Mb |
(ftp)(http) |
TSV |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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