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Series GSE234671 Query DataSets for GSE234671
Status Public on Apr 16, 2024
Title MNase-Seq analysis of wild-type Candida glabrata strain in RPMI-growth and macrophage-internalized conditions at 2 h and 10 h post-infection.
Organism Nakaseomyces glabratus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary MNase-sequencing analysis led to identification of 56,637 to 71,823 nucleosomes under RPMI-growth or macrophage-internalized conditions. Mapping of dynamic nucleosomes revealed that 12 to 24% of total nucleosomes were altered in their position, occupancy or fuzziness. Notably, while the nucleosome position shift was the most common dynamic event, the nucleosome fuzziness was the least observed change. Promoter regions were found to be nucleosome-depleted.
Overall design The study was designed to delineate the effect of the macrophage internal milieu on the chromatin dynamics in C. glabrata. C. glabrata wild-type cells were grown either in RPMI medium or infected to human THP-1 macrophages. After 2 h and 10 h, C. glabrata cells were collected, and chromatin structure was analysed via MNase-Sequencing approach. The experiment was performed with two biological replicates.
Contributor(s) Kumar K, Pareek A, Kaur R
Citation(s) 38632999
Submission date Jun 12, 2023
Last update date May 01, 2024
Contact name Rupinder Kaur
Organization name Centre for DNA Fingerprinting and Diagnostics
Lab Laboratory of Fungal Pathogenesis
Street address Inner ring road, Uppal
City Hyderabad
State/province Telangana
ZIP/Postal code 500039
Country India
Platforms (1)
GPL33483 Illumina HiSeq 2500 (Nakaseomyces glabratus)
Samples (8)
GSM7473129 KK-1: Macrophage internalized for 10 h; Replicate-1
GSM7473130 KK-1: Macrophage internalized for 10 h; Replicate-2
GSM7473131 KK-2: RPMI-grown for 10 h; Replicate-1
This SubSeries is part of SuperSeries:
GSE234809 SWI/SNF complex-mediated chromatin remodelling in Candida glabrata is vital for immune evasion
BioProject PRJNA982736

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE234671_KK1.txt.gz 1.4 Mb (ftp)(http) TXT
GSE234671_KK2.txt.gz 1.3 Mb (ftp)(http) TXT
GSE234671_KK3.txt.gz 1.6 Mb (ftp)(http) TXT
GSE234671_KK4.txt.gz 1.3 Mb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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