|
Status |
Public on Aug 28, 2024 |
Title |
Disturbed glycolipid metabolism activates CXCL13-CXCR5 axis in senescent TSCs to promote heterotopic ossification formation |
Organism |
Mus musculus |
Experiment type |
Expression profiling by high throughput sequencing
|
Summary |
Heterotopic ossification (HO) occurs as a common complication after injury, while its risk factor and mechanism remain unclear yet, which restricts the development of pharmacological treatment. Clinical research suggested that diabetes mellitus (DM) patients were prone to develop HO in tendon, but the solid evidence and mechanical research are still needed. Here, we combined the clinical samples and DM mice model to identify that disordered glycolipid metabolism aggravates the senescence of tendon derived stem cells (TSCs) and promotes their osteogenic differentiation. Then, combining the RNA-seq results of aging tendon, we detected the abnormally activated autocrine CXCL13-CXCR5 axis in TSCs cultured in high fat high glucose (HFHG) environment and also in aged tendon. Genetic inhibition of CXCL13 successfully alleviates the HO formation in DM mice, providing a potential therapeutical target for suppressing HO formation in DM patients after trauma or surgery.
|
|
|
Overall design |
RNA-seq using RNA extracted from TSCs cultured in HFHG medium or LG medium
|
|
|
Contributor(s) |
Chen Y, Zhou H |
Citation(s) |
38880863 |
|
Submission date |
Jun 14, 2023 |
Last update date |
Aug 28, 2024 |
Contact name |
Yuyu Chen |
E-mail(s) |
chenyy225@mail2.sysu.edu.cn
|
Phone |
+8618819473165
|
Organization name |
Sun Yat-sen University
|
Street address |
Zhongshan II road, 74th
|
City |
Guangzhou |
State/province |
Guangdong |
ZIP/Postal code |
510080 |
Country |
China |
|
|
Platforms (1) |
GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
|
Samples (8)
|
|
Relations |
BioProject |
PRJNA983738 |