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Series GSE234916 Query DataSets for GSE234916
Status Public on Aug 28, 2024
Title Disturbed glycolipid metabolism activates CXCL13-CXCR5 axis in senescent TSCs to promote heterotopic ossification formation
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Heterotopic ossification (HO) occurs as a common complication after injury, while its risk factor and mechanism remain unclear yet, which restricts the development of pharmacological treatment. Clinical research suggested that diabetes mellitus (DM) patients were prone to develop HO in tendon, but the solid evidence and mechanical research are still needed. Here, we combined the clinical samples and DM mice model to identify that disordered glycolipid metabolism aggravates the senescence of tendon derived stem cells (TSCs) and promotes their osteogenic differentiation. Then, combining the RNA-seq results of aging tendon, we detected the abnormally activated autocrine CXCL13-CXCR5 axis in TSCs cultured in high fat high glucose (HFHG) environment and also in aged tendon. Genetic inhibition of CXCL13 successfully alleviates the HO formation in DM mice, providing a potential therapeutical target for suppressing HO formation in DM patients after trauma or surgery.
 
Overall design RNA-seq using RNA extracted from TSCs cultured in HFHG medium or LG medium
 
Contributor(s) Chen Y, Zhou H
Citation(s) 38880863
Submission date Jun 14, 2023
Last update date Aug 28, 2024
Contact name Yuyu Chen
E-mail(s) chenyy225@mail2.sysu.edu.cn
Phone +8618819473165
Organization name Sun Yat-sen University
Street address Zhongshan II road, 74th
City Guangzhou
State/province Guangdong
ZIP/Postal code 510080
Country China
 
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (8)
GSM7476454 HFHG_1
GSM7476455 HFHG_2
GSM7476456 HFHG_3
Relations
BioProject PRJNA983738

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE234916_FPKM_anno.xls.gz 3.9 Mb (ftp)(http) XLS
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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