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| Status |
Public on Aug 09, 2010 |
| Title |
Comparative transcriptomic profiling of peripheral blood mononuclear cells (PBMNCs) isolated from pigs exposed to a high-fat diet (H-F) or to a high-fat diet plus resveratrol (H-F+RES) |
| Organism |
Sus scrofa |
| Experiment type |
Expression profiling by array
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| Summary |
We used Affymetrix microarrays to investigate gene expression changes in PBMNCs isolated from female and male pigs to determine significant modulatory effects that may have been induced by the intake of resveratrol during 9 months in high-fat fed animals .
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| Overall design |
The aim of this work was to determine whether the intake of dietary levels of resveratrol (RES) exerted any modulatory effects, at the level of gene expression, in PBMNCs isolated from female and male pigs exposed to a H-F diet for 9 months. We first isolated PBMNCs, and the corresponding total RNA, from 5 female pigs and 5 male pigs at time 0 (beginning of the experiment) to determine basal gene expression levels and to compare sex differential gene expression. Animals designated to the control group were then fed a normal chow (5% beef tallow) for 9 months. Animals in the H-F diet were fed a chow with 20% of beef tallow and animals in the H-F diet supplemented with resveratrol were fed the 20% fat diet plus resveratrol (8 mg/Kg body weight per day). From each group, PBMNCs were isolated from 2 female and 2 male animals. Pure RNA was extracted from the PBMNCs for microarrays analyses (Affymetrix) and gene differential expression determined between the H-F fed animals and CT animals (to determine the effects of fat consumption) and between the H-F fed animals supplemented with RES and the H-F group (to determine the effects of RES on the fat consumption).
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| Contributor(s) |
García-Conesa M, Azorín-Ortuño M, Espín de Gea J |
| Citation(s) |
21852083 |
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| Submission date |
Aug 08, 2010 |
| Last update date |
May 03, 2013 |
| Contact name |
María-Teresa García-Conesa |
| E-mail(s) |
mtconesa@cebas.csic.es
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| Phone |
+ 34 968 396276
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| Organization name |
CEBAS-CSIC
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| Department |
Quality, Safety and Bioactivity of Plant Foods
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| Street address |
Campus de Espinardo
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| City |
Espinardo |
| State/province |
Murcia |
| ZIP/Postal code |
30100 |
| Country |
Spain |
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| Platforms (1) |
| GPL3533 |
[Porcine] Affymetrix Porcine Genome Array |
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| Samples (22)
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| GSM575712 |
Control group, Time 0 (basal), female pig number 6 |
| GSM575713 |
Control group, Time 0 (basal), female pig number 8 |
| GSM575714 |
Control group, Time 0 (basal), female pig number 10 |
| GSM575715 |
Control group, Time 0 (basal), female pig number 17 |
| GSM575716 |
Control group, Time 0 (basal), female pig number 20 |
| GSM575717 |
Control group, Time 0 (basal), male pig number 11 |
| GSM575718 |
Control group, Time 0 (basal), male pig number 12 |
| GSM575719 |
Control group, Time 0 (basal), male pig number 15 |
| GSM575720 |
Control group, Time 0 (basal), male pig number 24 |
| GSM575721 |
Control group, Time 0 (basal), male pig number 25 |
| GSM575722 |
Control group, Time 9 months, female pig number 18 |
| GSM575723 |
Control group, Time 9 months, female pig number 8 |
| GSM575724 |
Control group, Time 9 months, male pig number 16 |
| GSM575725 |
Control group, Time 9 months, male pig number 25 |
| GSM575726 |
High-fat fed group, Time 9 months, female pig number 27 |
| GSM575727 |
High-fat fed group, Time 9 months, female pig number 30 |
| GSM575728 |
High-fat fed group, Time 9 months, male pig number 23 |
| GSM575729 |
High-fat fed group, Time 9 months, male pig number 26 |
| GSM575730 |
High-fat+ resveratrol fed group, Time 9 months, female pig number 21 |
| GSM575731 |
High-fat+ resveratrol fed group, Time 9 months, female pig number 22 |
| GSM575732 |
High-fat+ resveratrol fed group, Time 9 months, male pig number 4 |
| GSM575733 |
High-fat+ resveratrol fed group, Time 9 months, male pig number 5 |
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| Relations |
| BioProject |
PRJNA131073 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE23503_RAW.tar |
43.3 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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