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| Status |
Public on Aug 11, 2010 |
| Title |
Effect of hIAPP aggregation on gene expression by mouse bone marrow-derived macrophages after 12 hours |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
The purpose of this experiment was to determine changes in gene expression by bone marrow-derived macrophages (BMDMs) treated with synthetic human vs. rodent islet amyloid polypeptide (IAPP). Synthetic human IAPP at 15 uM aggregates to form fibrils in vitro, whereas rodent IAPP is non-amyloidogenic. We hypothesized that interaction of macrophages with human IAPP aggregates can activate pro-inflammatory signalling pathways in macrophages, as described for other amyloidogenic peptides.
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| Overall design |
This array includes eight samples from one experiment, with two groups of four replicates each. Each replicate represents BMDMs from one well of a 24-well plate. The control group was treated with 15 uM rat IAPP for 12 hours prior to total RNA extraction; the experimental group was treated with 15 uM human IAPP.
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| Contributor(s) |
Westwell-Roper C, Verchere CB |
| Citation(s) |
21813778 |
| Submission date |
Aug 10, 2010 |
| Last update date |
Jun 14, 2018 |
| Contact name |
Clara Westwell-Roper |
| E-mail(s) |
cwestwel@interchange.ubc.ca
|
| Phone |
604-875-3792
|
| Organization name |
Child and Family Research Institute, University of British Columbia
|
| Department |
Pathology and Laboratory Medicine
|
| Lab |
Dr. C. Bruce Verchere
|
| Street address |
950 West 28th Ave
|
| City |
Vancouver |
| State/province |
BC |
| ZIP/Postal code |
V5Z 4H4 |
| Country |
Canada |
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| Platforms (1) |
| GPL6885 |
Illumina MouseRef-8 v2.0 expression beadchip |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA131753 |
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