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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Jun 19, 2024 |
| Title |
Loss of LPAR6 and CAB39L Dysregulate Basal to Luminal Urothelial Differentiation Program Contributing to Bladder Carcinogenesis |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
In vitro and in vivo studies including single cell sequencing showed that LPAR6 and CAB39L play a major role in regulating basal to luminal urothelial differentiation. Their loss of function contributed to bladder carcinogenesis by dysregulating urothelial differentiation program and sensitizing the urothelium to N-butyl-N-(4-hydroxybutyl)nitrosamine (BBN) induced cancers, which recapitulated luminal and basal subtypes of human bladder cancer.
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| Overall design |
Single cell sequencing of the bladder urothelium was performed on cells harvested from wild type, Lpar6 knockout and Cab39l knockout mice. For each group a pooled sample of single urothelial cell suspension harvested from 15 mice was prepared.
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| Contributor(s) |
Lee S, Bondaruk J, Wang Y, Chen H, Lee JG, Majewski T, Mullen RD, Cogdell D, Chen J, Wang Z, Yao H, Kus P, Jeong J, Lee I, Choi W, Navai N, Guo C, Dinney C, Baggerly K, Mendelsohn C, McConkey D, Behringer RR, Kimmel M, Wei P, Czerniak B |
| Citation(s) |
38676926 |
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| Submission date |
Jun 21, 2023 |
| Last update date |
Oct 16, 2024 |
| Contact name |
Bogdan A Czerniak |
| Organization name |
UT MD Anderson Cancer Center
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| Department |
Pathology
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| Lab |
Czerniak
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| Street address |
1515 Holcombe
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| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77030 |
| Country |
USA |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (3) |
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| Relations |
| BioProject |
PRJNA986096 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE235455_RAW.tar |
219.1 Mb |
(http)(custom) |
TAR (of MTX, TSV) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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