|
| Status |
Public on Mar 15, 2024 |
| Title |
Differential expression analysis of Aspergillus niger genes in mRMA level between in seed medium and fermentation media for L-malic acid production |
| Organism |
Aspergillus niger |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Generally, A. niger undergoes two steps consisting of seed stage and fermentation stage to synthesize malic acid. However, it remains unknown which metabolic pathways, regulators, and key genes respond to the transition from seed medium to fermentation medium. In this study, we obtained the total mRMA of a high L-malate-yielding strain A. niger RG0095 at seed stage and fermentation stage, respectively, and performed a global differential expression analysis.
|
| |
|
| Overall design |
To investigate the mRNA expression changes of RG0095 genes between seed culture and fermentation culture, the fresh RG0095 spores were cultured in seed medium for 20 h and then the resulting mycelium were transferred into fermentation medium for 72 h. The gene expression profiling analysis using data obtained from RNA-seq of one strain at 2 time points. Comparative gene expression profiling analysis of RNA-seq data for RG0095 stain in two culture state at 2 time points.
|
| |
|
| Contributor(s) |
Zhang C, Zhang T, Xu Q |
| Citation(s) |
38506527 |
| |
| Submission date |
Jun 21, 2023 |
| Last update date |
Jun 15, 2024 |
| Contact name |
Chi Zhang |
| E-mail(s) |
zhangchi@njnu.edu.cn
|
| Phone |
8618795955583
|
| Organization name |
Nanjing Normal University
|
| Department |
School of Food Science and Pharmaceutical Engineering
|
| Lab |
Chi Zhang
|
| Street address |
1 WenYuan Road
|
| City |
NanJing |
| State/province |
Jiangsu |
| ZIP/Postal code |
210023 |
| Country |
China |
| |
|
| Platforms (1) |
| GPL32288 |
Illumina NovaSeq 6000 (Aspergillus niger) |
|
| Samples (6)
|
| GSM7504769 |
RG0095 control seed 20 h biol rep 1 [A_1] |
| GSM7504770 |
RG0095 control seed 20 h biol rep 2 [A_2] |
| GSM7504771 |
RG0095 control seed 20 h biol rep 3 [A_3] |
| GSM7504772 |
RG0095 fermentation 72 h biol rep 1 [C_1] |
| GSM7504773 |
RG0095 fermentation 72 h biol rep 2 [C_2] |
| GSM7504774 |
RG0095 fermentation 72 h biol rep 3 [C_3] |
|
| Relations |
| BioProject |
PRJNA986226 |
Less...
More...