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| Status |
Public on Jun 01, 2024 |
| Title |
Impact of shear stretch and oxygen toxicity on pulmonary MSC of the preterm infant. |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Bronchopulmonary dysplasia (BPD) represents one of the most important sequelae of preterm birth affecting more than 20% of infants born below 32 weeks. Mechanical ventilation exerting shear stress (cyclic mechanical stress, CMS) and oxygen toxicity (hyperoxia, Hox) mainly contribute to the pathogenesis of BPD. The study proposal aims to separate the impact of CMS and Hox on pulmonary mesenchymal stem cells (MSC) isolated from preterm infants participating in the DZL PROTECT-AIRR cohort study in a world-wide unique in vitro setting. The impact of rhythmic 2D and 3D stretch to MSC cell cultures alone and in combination with different fractions of oxygen is evaluated with respect to MSC phenotype, transcriptional regulation, protein alterations and paracrine secretion.
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| Overall design |
6 groups with 8-16 biological replicates
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| Contributor(s) |
Wilhelm J, Behnke J, Erhardt H |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Jun 22, 2023 |
| Last update date |
Jun 01, 2024 |
| Contact name |
Jochen Wilhelm |
| Organization name |
Justus-Liebig University Giessen
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| Department |
Institute for Lung Health
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| Lab |
ILH Genomics
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| Street address |
Aulweg 132
|
| City |
Giessen |
| State/province |
Hessen |
| ZIP/Postal code |
35392 |
| Country |
Germany |
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| Platforms (1) |
| GPL20844 |
Agilent-072363 SurePrint G3 Human GE v3 8x60K Microarray 039494 [Feature Number Version] |
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| Samples (64)
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| Relations |
| BioProject |
PRJNA986473 |
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