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| Status |
Public on Jun 01, 2024 |
| Title |
Structure of an aberrant spliceosome intermediate on its way to disassembly |
| Organism |
Schizosaccharomyces pombe |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Intron removal during pre-mRNA splicing is of extraordinary complexity and its disruption causes a vast number of genetic diseases in humans. While key steps of the canonical spliceosome cycle have been revealed by combined structure-function analyses, structural information on an aberrant spliceosome committed to discard is not available. Here, we report the cryo-EM structure of a B state spliceosome intermediate primed for disassembly. We identify the DEAH-box helicase – G patch protein pair (Gih35-Gpl1) to maintain catalytic dormancy with Gpl1 recognizing a remodeled active site of the spliceosome due to a single-nucleotide insertion at the 3’ end of the 5’ exon. Remodeling is communicated to the spliceosome surface and the Ntr1 complex is recruited. Our data pave the way for a targeted analysis of spliceosome-associated quality control.
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| Overall design |
Detailed analysis of Ctr1-deficient and WT strains using high throughput poly(A)+ RNA sequencing.
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| Contributor(s) |
Horvath A, Fischer T, Soni K |
| Citation missing |
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| |
| Submission date |
Jun 22, 2023 |
| Last update date |
Jun 01, 2024 |
| Contact name |
Attila Horvath |
| E-mail(s) |
attila.horvath@anu.edu.au
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| Organization name |
The Australian National University
|
| Street address |
Garran Road 131
|
| City |
Canberra |
| State/province |
ACT |
| ZIP/Postal code |
2601 |
| Country |
Australia |
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| Platforms (1) |
| GPL28961 |
Illumina NovaSeq 6000 (Schizosaccharomyces pombe) |
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| Samples (3) |
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| Relations |
| BioProject |
PRJNA986513 |
