 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Jan 08, 2024 |
| Title |
Effects of lifelong exercise and age on transcriptome dynamics in MHC I and MHC IIa muscle fibers at baseline and in response to resistance exercise |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
RNA-Seq was used for the first time to investigate the effects of resistance exercise on transcriptome dynamics in slow myosin heavy chain (MHC) I and fast MHC IIa muscle fibers among 3 groups of men. Vastus lateralis muscle biopsies were obtained before and 4 hours after resistance exercise.
|
| |
|
| Overall design |
A total of 25 males were included in this investigation. The participants included 8 lifelong exercisers (74±1y), 9 old healthy non-exercisers (75±1y), and 8 young exercisers (25±1y). Vastus lateralis biopsies were obtained in conjunction with a resistance exercise bout (bilateral knee extensions with 3x10 reps at 70% of 1-RM) and included a basal biopsy and another biopsy 4 hours after the exercise session. From each biopsy sample, individual muscle fibers were isolated. After myosin isoform identification of isolated fibers via SDS-PAGE, RNA extraction of MHC I and MHC IIa muscle fibers was performed. Thus, each resulting sample contained total RNA from either MHC I or MHC IIa muscle fibers. A total of 100 samples were analyzed using RNA-Seq. The study design allowed us to examine the acute effects of resistance exercise on transcriptome dynamics in MHC I and MHC IIa muscle fibers with age and exercise.
|
| |
|
| Contributor(s) |
Raue U, Begue G, Minchev K, Jemiolo B, Gries KJ, Chambers TL, Rubenstein AB, Zaslavsky E, Sealfon SC, Trappe TA, Trappe S |
| Citation(s) |
38095016 |
| |
| Submission date |
Jun 26, 2023 |
| Last update date |
Jan 30, 2024 |
| Contact name |
Aliza Rubenstein |
| E-mail(s) |
aliza.rubenstein@mssm.edu
|
| Organization name |
Icahn School of Medicine at Mount Sinai
|
| Street address |
1 Gustave L. Levy Pl
|
| City |
Manhattan |
| State/province |
NY |
| ZIP/Postal code |
10029 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
|
| Samples (81)
|
| GSM7509418 |
vastus lateralis muscle,SLOW,LLE,trained,1,male,PRE |
| GSM7509419 |
vastus lateralis muscle,SLOW,LLE,trained,1,male,POST |
| GSM7509420 |
vastus lateralis muscle,FAST,LLE,trained,1,male,PRE |
| GSM7509421 |
vastus lateralis muscle,FAST,LLE,trained,1,male,POST |
| GSM7509422 |
vastus lateralis muscle,SLOW,LLE,trained,2,male,PRE |
| GSM7509423 |
vastus lateralis muscle,SLOW,LLE,trained,2,male,POST |
| GSM7509424 |
vastus lateralis muscle,FAST,LLE,trained,2,male,PRE |
| GSM7509425 |
vastus lateralis muscle,FAST,LLE,trained,2,male,POST |
| GSM7509426 |
vastus lateralis muscle,SLOW,LLE,trained,3,male,PRE |
| GSM7509427 |
vastus lateralis muscle,SLOW,LLE,trained,3,male,POST |
| GSM7509428 |
vastus lateralis muscle,FAST,LLE,trained,3,male,PRE |
| GSM7509429 |
vastus lateralis muscle,FAST,LLE,trained,3,male,POST |
| GSM7509430 |
vastus lateralis muscle,SLOW,LLE,trained,4,male,PRE |
| GSM7509431 |
vastus lateralis muscle,SLOW,LLE,trained,4,male,POST |
| GSM7509432 |
vastus lateralis muscle,FAST,LLE,trained,4,male,PRE |
| GSM7509433 |
vastus lateralis muscle,FAST,LLE,trained,4,male,POST |
| GSM7509434 |
vastus lateralis muscle,SLOW,LLE,trained,5,male,PRE |
| GSM7509435 |
vastus lateralis muscle,SLOW,LLE,trained,5,male,POST |
| GSM7509436 |
vastus lateralis muscle,FAST,LLE,trained,5,male,PRE |
| GSM7509437 |
vastus lateralis muscle,FAST,LLE,trained,5,male,POST |
| GSM7509438 |
vastus lateralis muscle,SLOW,LLE,trained,7,male,PRE |
| GSM7509439 |
vastus lateralis muscle,SLOW,LLE,trained,7,male,POST |
| GSM7509440 |
vastus lateralis muscle,FAST,LLE,trained,7,male,PRE |
| GSM7509441 |
vastus lateralis muscle,FAST,LLE,trained,7,male,POST |
| GSM7509442 |
vastus lateralis muscle,SLOW,LLE,trained,8,male,PRE |
| GSM7509443 |
vastus lateralis muscle,SLOW,LLE,trained,8,male,POST |
| GSM7509444 |
vastus lateralis muscle,FAST,LLE,trained,8,male,PRE |
| GSM7509445 |
vastus lateralis muscle,FAST,LLE,trained,8,male,POST |
| GSM7509446 |
vastus lateralis muscle,SLOW,LLE,trained,9,male,PRE |
| GSM7509447 |
vastus lateralis muscle,SLOW,LLE,trained,9,male,POST |
| GSM7509448 |
vastus lateralis muscle,FAST,LLE,trained,9,male,PRE |
| GSM7509449 |
vastus lateralis muscle,FAST,LLE,trained,9,male,POST |
| GSM7509450 |
vastus lateralis muscle,SLOW,YE,trained,1,male,PRE |
| GSM7509451 |
vastus lateralis muscle,SLOW,YE,trained,1,male,POST |
| GSM7509452 |
vastus lateralis muscle,FAST,YE,trained,1,male,PRE |
| GSM7509453 |
vastus lateralis muscle,FAST,YE,trained,1,male,POST |
| GSM7509454 |
vastus lateralis muscle,SLOW,YE,trained,2,male,PRE |
| GSM7509455 |
vastus lateralis muscle,SLOW,YE,trained,2,male,POST |
| GSM7509456 |
vastus lateralis muscle,FAST,YE,trained,2,male,PRE |
| GSM7509457 |
vastus lateralis muscle,FAST,YE,trained,2,male,POST |
| GSM7509458 |
vastus lateralis muscle,SLOW,YE,trained,3,male,PRE |
| GSM7509459 |
vastus lateralis muscle,SLOW,YE,trained,3,male,POST |
| GSM7509460 |
vastus lateralis muscle,FAST,YE,trained,3,male,PRE |
| GSM7509461 |
vastus lateralis muscle,FAST,YE,trained,3,male,POST |
| GSM7509462 |
vastus lateralis muscle,SLOW,YE,trained,4,male,PRE |
| GSM7509463 |
vastus lateralis muscle,SLOW,YE,trained,4,male,POST |
| GSM7509464 |
vastus lateralis muscle,FAST,YE,trained,4,male,PRE |
| GSM7509465 |
vastus lateralis muscle,FAST,YE,trained,4,male,POST |
| GSM7509466 |
vastus lateralis muscle,SLOW,YE,trained,5,male,POST |
| GSM7509467 |
vastus lateralis muscle,FAST,YE,trained,5,male,PRE |
| GSM7509468 |
vastus lateralis muscle,FAST,YE,trained,5,male,POST |
| GSM7509469 |
vastus lateralis muscle,SLOW,YE,trained,6,male,PRE |
| GSM7509470 |
vastus lateralis muscle,SLOW,YE,trained,6,male,POST |
| GSM7509471 |
vastus lateralis muscle,FAST,YE,trained,6,male,PRE |
| GSM7509472 |
vastus lateralis muscle,FAST,YE,trained,6,male,POST |
| GSM7509473 |
vastus lateralis muscle,SLOW,YE,trained,7,male,PRE |
| GSM7509474 |
vastus lateralis muscle,SLOW,YE,trained,7,male,POST |
| GSM7509475 |
vastus lateralis muscle,FAST,YE,trained,7,male,PRE |
| GSM7509476 |
vastus lateralis muscle,FAST,YE,trained,7,male,POST |
| GSM7509477 |
vastus lateralis muscle,SLOW,YE,trained,8,male,PRE |
| GSM7509478 |
vastus lateralis muscle,SLOW,YE,trained,8,male,POST |
| GSM7509479 |
vastus lateralis muscle,FAST,YE,trained,8,male,PRE |
| GSM7509480 |
vastus lateralis muscle,FAST,YE,trained,8,male,POST |
| GSM7509481 |
vastus lateralis muscle,SLOW,OH,untrained,1,male,POST |
| GSM7509482 |
vastus lateralis muscle,FAST,OH,untrained,1,male,POST |
| GSM7509483 |
vastus lateralis muscle,SLOW,OH,untrained,2,male,POST |
| GSM7509484 |
vastus lateralis muscle,FAST,OH,untrained,2,male,POST |
| GSM7509485 |
vastus lateralis muscle,SLOW,OH,untrained,3,male,POST |
| GSM7509486 |
vastus lateralis muscle,FAST,OH,untrained,3,male,POST |
| GSM7509487 |
vastus lateralis muscle,SLOW,OH,untrained,4,male,POST |
| GSM7509488 |
vastus lateralis muscle,FAST,OH,untrained,4,male,POST |
| GSM7509489 |
vastus lateralis muscle,SLOW,OH,untrained,5,male,POST |
| GSM7509490 |
vastus lateralis muscle,FAST,OH,untrained,5,male,POST |
| GSM7509491 |
vastus lateralis muscle,SLOW,OH,untrained,6,male,POST |
| GSM7509492 |
vastus lateralis muscle,FAST,OH,untrained,6,male,POST |
| GSM7509493 |
vastus lateralis muscle,SLOW,OH,untrained,7,male,POST |
| GSM7509494 |
vastus lateralis muscle,FAST,OH,untrained,7,male,POST |
| GSM7509495 |
vastus lateralis muscle,SLOW,OH,untrained,8,male,POST |
| GSM7509496 |
vastus lateralis muscle,FAST,OH,untrained,8,male,POST |
| GSM7509497 |
vastus lateralis muscle,SLOW,OH,untrained,9,male,POST |
| GSM7509498 |
vastus lateralis muscle,FAST,OH,untrained,9,male,POST |
|
| This SubSeries is part of SuperSeries: |
| GSE236600 |
Effects of lifelong exercise and age on transcriptome dynamics in MHC I and MHC IIa muscle fibers at baseline and in response to resistance exercise (all samples) |
|
| Relations |
| BioProject |
PRJNA987612 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE235781_counts_geo.txt.gz |
7.1 Mb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
|
|
|
|
 |
Less...
More...