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| Status |
Public on May 22, 2024 |
| Title |
Macrophages modulate fibrosis during newt lens regeneration [PWaltl] |
| Organism |
Pleurodeles waltl |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Previous studies indicated that macrophages play a role during lens regeneration in newts, but their function has not been tested experimentally. Here we generated a transgenic newt reporter line in which macrophages can be visualized in vivo. Using this new tool, we analyzed the location of macrophages during lens regeneration. We uncovered early gene expression changes using bulk RNAseq in two newt species, Notophthalmus viridescens and Pleurodeles waltl. Next, we used clodronate liposomes to deplete macrophages, which inhibited lens regeneration in both newt species. Macrophage depletion induced the formation of scar-like tissue, an increased and sustained inflammatory response, an early decrease in iris pigment epithelial cell (iPEC) proliferation and a late increase in apoptosis. Some of these phenotypes persisted for at least 100 days and could be rescued by exogenous FGF2. Re-injury alleviated the effects of macrophage depletion and re-started the regeneration process. Together, our findings highlight the importance of macrophages in facilitating a pro-regenerative environment in the newt eye, helping to resolve fibrosis, modulating the overall inflammatory landscape and maintaining the proper balance of early proliferation and late apoptosis.
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| Overall design |
Bulk RNA-seq was performed using dorsal iris tissue from Pleurodeles waltl over a time course of lens regeneration, including from the intact iris, 1 day post-lentectomy and 4 days post-lentectomy.
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| Contributor(s) |
Tsissios G, Sallese A, Perez-Estrada JR, Tangeman JA, Chen W, Smucker B, Ratvasky SC, Grajales-Esquivel E, Martinez A, Visser KJ, Araus AJ, Wang H, Simon A, Yun MH, Del Rio-Tsonis K |
| Citation(s) |
38745238 |
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| Submission date |
Jul 10, 2023 |
| Last update date |
May 22, 2024 |
| Contact name |
Katia Del Rio-Tsonis |
| E-mail(s) |
delriok@miamioh.edu
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| Organization name |
Miami University
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| Department |
Biology
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| Street address |
700 E High St
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| City |
Oxford |
| State/province |
OH |
| ZIP/Postal code |
45056 |
| Country |
USA |
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| Platforms (1) |
| GPL32009 |
Illumina NovaSeq 6000 (Pleurodeles waltl) |
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| Samples (9)
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| GSM7585131 |
Iris,Dorsal,1dpl,Rep1 [1dpl_1] |
| GSM7585132 |
Iris,Dorsal,1dpl,Rep2 [1dpl_2] |
| GSM7585133 |
Iris,Dorsal,1dpl,Rep3 [1dpl_3] |
| GSM7585134 |
Iris,Dorsal,4dpl,Rep1 [4dpl_1] |
| GSM7585135 |
Iris,Dorsal,4dpl,Rep2 [4dpl_2] |
| GSM7585136 |
Iris,Dorsal,4dpl,Rep3 [4dpl_3] |
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| This SubSeries is part of SuperSeries: |
| GSE236908 |
Macrophages modulate fibrosis during newt lens regeneration. |
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| Relations |
| BioProject |
PRJNA993070 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE236906_PWaltl_transcriptome.fasta.gz |
77.1 Mb |
(ftp)(http) |
FASTA |
| GSE236906_avgTPM.csv.gz |
9.9 Mb |
(ftp)(http) |
CSV |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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