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Series GSE237259 Query DataSets for GSE237259
Status Public on Jul 23, 2023
Title high efficiency and multiplexable Strand specificity RNA sequencing by Double Adapter Directional Capture
Organisms Mycolicibacterium smegmatis; Homo sapiens; Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary The quantification of transcriptome through RNA sequencing (RNA-seq) has widely been used to characterize all patterns of genes in a certain period. However, although many experimental protocols have recently been developed, RNA-seq is still a challenging technology in application. It is necessary to develop an easy and versatile RNA-seq method available in a wide range from single cell RNA to bulk RNA. Here we introduce a novel RNA-seq method, Double Adapter Directional Capture sequencing (DADCSeq), by directly adding adaptor at both termini of library respectively in the steps of reverse transcription and second strand synthesis, which can not only produce high quality strand specificity full-length RNA-seq library but also reduces cockamamie steps of library constructions with less time consuming. The DADCSeq was also optimized for prokaryote such as Mycobacterium smegmatis, a model bacterium for Mycobacterium tuberculosis, and can detect more genes compared to traditional methods. Taken together the DADCSeq offers a greatly simplified and cost-effective protocol with high repeatable strand specificity RNA-seq results for a diversity of RNA input materials from prokaryote to eucaryote RNA-seq libraries.
 
Overall design To develop an easy and versatile RNA-seq method available in RNA library
 
Contributor(s) Sun Z, He B
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Submission date Jul 13, 2023
Last update date Jul 24, 2023
Contact name zaiqiao sun
E-mail(s) 2019102040019@whu.edu.cn
Phone +8616620125505
Organization name wuhan university
Street address No.299
City wuhan
ZIP/Postal code 430072
Country China
 
Platforms (3)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
GPL28556 Illumina NovaSeq 6000 (Mycolicibacterium smegmatis)
Samples (80)
GSM7597908 Mycobacterium smegmatis, RNAseq library by DADCSeq repeat1
GSM7597909 Mycobacterium smegmatis, RNAseq library by DADCSeq repeat2
GSM7597910 Mycobacterium smegmatis, RNAseq library by DADCSeq repeat3
Relations
BioProject PRJNA994488

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE237259_HS_DADCvsSHERRYvsHieff_matrix.txt.gz 5.7 Mb (ftp)(http) TXT
GSE237259_HS_optimize_matrix.txt.gz 6.3 Mb (ftp)(http) TXT
GSE237259_MM_DWTvsDRaf_matrix.txt.gz 3.7 Mb (ftp)(http) TXT
GSE237259_MM_HWTvsHRaf_matrix.txt.gz 3.7 Mb (ftp)(http) TXT
GSE237259_MM_Spleen_matrix.txt.gz 4.1 Mb (ftp)(http) TXT
GSE237259_MS_DADCvsHieff_matrix.txt.gz 130.8 Kb (ftp)(http) TXT
GSE237259_RAW.tar 353.5 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
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