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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Mar 21, 2024 |
| Title |
Expression profile of shMCT-1 in 4T1 metastatic murine triple-negative breast cancer (TNBC) cell lines in response to anti-IL6R immunotherapy |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Malignant T-cell amplified sequence (MCT-1 or MCTS1) is an oncoprotein that regulates translation re-initiation, ribosomal recycling, and tumorigenicity. Given that MCT-1 amplifies interleukin-6 (IL-6) trans-signaling by elevating interaction between interleukin-6 receptor (IL-6R) and glycoprotein 130 (gp130), we aimed to dissect whether targeting IL-6R alters transcriptional profile of TNBC cells with different MCT-1 background. Through RNA-seq, we have characterized that anti-IL6R immunotherapy suppressed genes involved in cell proliferation and metastatic cascades but enriched genes associated in cell adhesion in 4T1 murine TNBC cells with shMCT-1.
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| Overall design |
Samples include two independent biological replicates of the scramble cells and MCT-1 silencing (shMCT-1) cells treated with anti-IL6R
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| Web link |
https://www.thno.org/v14p2167.htm
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| Contributor(s) |
Hsu H |
| Citation(s) |
38505617 |
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| Submission date |
Jul 17, 2023 |
| Last update date |
Mar 22, 2024 |
| Contact name |
Hsin-Ling Hsu |
| E-mail(s) |
hsinling88@nhri.edu.tw
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| Phone |
(886)372-46166
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| Organization name |
National Health Research Institutes
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| Department |
Institute of Molecular and Genomic Medicine
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| Street address |
35 Keyan Road
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| City |
Zhunan |
| State/province |
Miaoli |
| ZIP/Postal code |
35053 |
| Country |
Taiwan |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (8)
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| GSM7622367 |
scramble, anti-IL6R, replicate 2 |
| GSM7622368 |
shMCT-1, IgG, replicate 1 |
| GSM7622369 |
shMCT-1, IgG, replicate 2 |
| GSM7622370 |
shMCT-1, anti-IL6R, replicate 1 |
| GSM7622371 |
shMCT-1, anti-IL6R, replicate 2 |
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| Relations |
| BioProject |
PRJNA995548 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE237506_RAW.tar |
1.8 Mb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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