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| Status |
Public on Mar 27, 2024 |
| Title |
Single cell sequencing data of islets from WT and Npc1KO mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Npc1 is a cholesterol transporter located on lysosomal membrane, and whether it is involved in regulating the function and differentiation of β cells is unclear. To understand molecular basis of the phenotype caused by Npc1 loss in β cells, we isolated islets from 2-week-old WT and Npc1KO mice for single-cell sequencing. We compared the gene expression profiles of different subpopulation of islet cells and found many changes of islet subpopulation and biological pathway occurred in Npc1KO islets.
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| Overall design |
To understand molecular basis of the phenotype caused by Npc1 loss in β cells, we isolated islets from 2-week-old WT and Npc1KO mice, digested the islet into single-cell suspension, and performed single-cell sequencing using 10X Genomics. Our islet samples were divided into two groups, WT and Npc1KO. Every islet suspension sample were digested by the islets of 3 same genotype 2-week-old female mice.
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| Contributor(s) |
Liu B, Gu Y |
| Citation(s) |
38505613 |
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| Submission date |
Jul 17, 2023 |
| Last update date |
Mar 28, 2024 |
| Contact name |
Bei Liu |
| E-mail(s) |
liubei066@sjtu.edu.cn
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| Phone |
18101917521
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| Organization name |
Ruijin Hospital, Shanghai Jiaotong University School of Medicine
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| Street address |
197 Rui Jin 2nd Road
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| City |
Shanghai |
| ZIP/Postal code |
200000 |
| Country |
China |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA995774 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE237585_RAW.tar |
144.3 Mb |
(http)(custom) |
TAR (of MTX, TSV) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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