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Series GSE23772 Query DataSets for GSE23772
Status Public on Aug 25, 2010
Title Why does HAMLET preferentially kill tumor cells? p38-dependent death in tumor but up-regulation of innate immunity in healthy, differentiated cells
Organism Homo sapiens
Experiment type Expression profiling by array
Summary HAMLET triggers a p38- and ER stress-dependent death response in carcinoma cells. Transcriptome and proteome analysis detected an increase in p38 expression and phosphorylation exclusively in carcinoma cells and p38 inhibitors delayed the death response to HAMLET in carcinoma and lymphoma cells. ER stress gene expression was also increased in tumor cells and HAMLET triggered rapid XBP1 mRNA splicing, eIF2a phosphorylation, and ATF6 cleavage as well as Hsc70 and CHOP activation, suggesting that ER stress caused by HAMLET may trigger p38 phosphorylation and death. The p38 inhibitor reduced the transcription of both p38 and ER stress gene transcription. Healthy differentiated cells, in contrast, showed no alteration in p38 signaling but a rapid innate immune response was detected and the cells survived HAMLET challenge.
 
Overall design 2 cell lines, time course, HAMLET treatment
 
Contributor(s) Storm P, Min S, Moreilhon C, Trulsson M, Chao Y, Westergren T, Gustafsson L, Aits S, Mossberg A, Ho J, Lim P, Yang H, Mok K, Svanborg C
Citation(s) 23505537
Submission date Aug 24, 2010
Last update date Oct 28, 2019
Contact name Henry Yang
E-mail(s) henry_yang@immunol.a-star.edu.sg
Organization name SIgN
Department Bioinformatics Group
Street address 8A Biomedical Grove
City Singapore
ZIP/Postal code 138648
Country Singapore
 
Platforms (1)
GPL6884 Illumina HumanWG-6 v3.0 expression beadchip
Samples (78)
GSM586718 A498 cells control 0min biological rep1
GSM586719 A498 cells control 0min biological rep2
GSM586720 A498 cells control 0min biological rep3
Relations
BioProject PRJNA130829

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE23772_RAW.tar 6.3 Mb (http)(custom) TAR
GSE23772_non-normalized.txt.gz 15.9 Mb (ftp)(http) TXT
Processed data included within Sample table
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