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| Status |
Public on Apr 02, 2024 |
| Title |
Novel insights into the role of the histone variant H2A.Z in the plant pathogen Fusarium fujikuroi [RNA-seq] |
| Organism |
Fusarium fujikuroi |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Transcriptome in respect to H2A.Z downregulation or overexpression
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| Overall design |
The F. fujikuroi IMI58289 wild-type strain (FfWT) provided by Commonwealth Mycological Institute, Kew, UK, was used as a parental strain to generate the H2A.Z knock-down strain (Tetoff::FfH2A.Z), the constitutive H2A.Z overexpression (OE::FfH2A.Z) strain as well as the N- and C-terminal hemagglutinin (HA)-tagged H2A.Z (HA::FfH2A.Z, FfH2A.Z::HA) strains. Submerged fungal growth was performed in 100 mL Darken pre-culture medium (Darken et al., 1959) on a rotary shaker at 180 rpm, 30°C in constant darkness for 3 days. Cultures inoculated with the TetOff::H2A.Z knock down strain were additionally supplemented with 0-50 µg/mL DOX to facilitate FfH2A.Z gene silencing. Flasks were incubated on a rotary shaker (180 rpm, 30°C) in the dark. Mycelia were harvested 3-4 days post inoculation (dpi) for RNA isolation. Isolation of fungal RNA was performed with lyophilized and ground mycelia from cultivation on solid CM or under liquid-standard conditions (ICI supplemented with 6 or 60 mM glutamine) for 3-4 days. Total RNA was extracted from samples, using the RNA reagent TRIzol (Thermo Fisher Scientific) as described in the manufacturer’s manual.
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| Contributor(s) |
K Atanasoff-Kardjalieff A, Berger H, Janevska S, Steinert K, Humpf H, Kalinina S, Strauss J, Ponts N, Studt-Reinhold L |
| Citation(s) |
38509556 |
| |
| Submission date |
Jul 19, 2023 |
| Last update date |
Apr 02, 2024 |
| Contact name |
Harald Berger |
| E-mail(s) |
harald.berger@symbiocyte.com
|
| Organization name |
Universität f. Bodenkultur Wien
|
| Department |
DAGZ
|
| Lab |
Strauss
|
| Street address |
Konrad-Lorenzstraße 43
|
| City |
Tulln |
| State/province |
NOE |
| ZIP/Postal code |
3430 |
| Country |
Austria |
| |
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| Platforms (1) |
| GPL33594 |
NextSeq 550 (Fusarium fujikuroi) |
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| Samples (10)
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| This SubSeries is part of SuperSeries: |
| GSE237765 |
Novel insights into the role of the histone variant H2A.Z in the plant pathogen Fusarium fujikuroi |
|
| Relations |
| BioProject |
PRJNA996566 |
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