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| Status |
Public on Apr 02, 2024 |
| Title |
Novel insights into the role of the histone variant H2A.Z in the plant pathogen Fusarium fujikuroi [ChIP-seq] |
| Organism |
Fusarium fujikuroi |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
ChIP-seq of histone PTM and H2A.Z depositioning in F. fujikuroi
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| |
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| Overall design |
The F. fujikuroi IMI58289 wild-type strain (FfWT) provided by Commonwealth Mycological Institute, Kew, UK, was used as a parental strain to generate the H2A.Z knock-down strain (Tetoff::FfH2A.Z), the constitutive H2A.Z overexpression (OE::FfH2A.Z) strain as well as the N- and C-terminal hemagglutinin (HA)-tagged H2A.Z (HA::FfH2A.Z, FfH2A.Z::HA) strains. Fungal mycelia were cultivated for three days in liquid ICI (6 mm glutamine) prior crosslinking with 1% formaldehyde. For immunoprecipitation, sonicated mycelia were incubated either with an anti-HA (Abcam, 9110), an anti-H3K27me3- (Active motif, 39155) or an anti-H3K4me3- (CellSignal, 9751) specific antibody. The assay was performed in biological duplicates, including input controls for every strain.
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| Contributor(s) |
K Atanasoff-Kardjalieff A, Berger H, Janevska S, Steinert K, Humpf H, Kalinina S, Strauss J, Ponts N, Studt-Reinhold L |
| Citation(s) |
38509556 |
| |
| Submission date |
Jul 19, 2023 |
| Last update date |
Apr 03, 2024 |
| Contact name |
Harald Berger |
| E-mail(s) |
harald.berger@symbiocyte.com
|
| Organization name |
Universität f. Bodenkultur Wien
|
| Department |
DAGZ
|
| Lab |
Strauss
|
| Street address |
Konrad-Lorenzstraße 43
|
| City |
Tulln |
| State/province |
NOE |
| ZIP/Postal code |
3430 |
| Country |
Austria |
| |
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| Platforms (1) |
| GPL33594 |
NextSeq 550 (Fusarium fujikuroi) |
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| Samples (13)
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| This SubSeries is part of SuperSeries: |
| GSE237765 |
Novel insights into the role of the histone variant H2A.Z in the plant pathogen Fusarium fujikuroi |
|
| Relations |
| BioProject |
PRJNA996567 |
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