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| Status |
Public on Jul 29, 2023 |
| Title |
RNA-Seq analyses in bta-miR-484 transfected adipocyte |
| Organism |
Bos taurus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
To elucidate the role of bta-miR-484 in adipocytes. we compared expression profiles of mRNAs in control and bta-miR-484 overexpressed adipocytes by RNA se-quencing. A total of 51 up-regulated genes and 77 down-regulated genes were detected in adipocytes overexpressing bta-miR-484. GO annotation showed that differ-entially expressed genes (DEGs） enrichment positive regulation of glycogen biosynthetic process, positive regulation of glucose import and other biological processes. COG classification found that DEGs are rich in Amino acid transport and metabolism, Posttranslational modification, protein turnover, chaperones, Secondary metabolites bio-synthesis, transport and catabolism, General function prediction only, Signal transduc-tion mechanisms, and other items. KEGG enrichment analysis showed that DEGs were enriched in apoptosis, AMPK signaling pathway, calcium signaling pathway, Ras signaling pathway, MAPK signaling pathway, Wnt signaling pathway, PI3K-Akt signaling pathway, adipocyto-kine signaling pathway, insulin secretion , PPAR signaling pathway, etc.
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| Overall design |
Adipocytes were isolated from bovine subcutaneous fat. One group was transfected with bta-miR-484 agomir, and the other group was transfected with bta-miR-484 agomir NC. Adipocytes transfected for 48h were collected for RNA extraction and analysis.
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| Contributor(s) |
Yang M, Gao X, Hu C, Wang S, Sheng H, Ma Y |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Jul 24, 2023 |
| Last update date |
Jul 29, 2023 |
| Contact name |
Mengli Yang |
| Organization name |
Ningxia University
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| Street address |
No. 489, Helan Mountain West Road, Xixia District, Yinchuan City, Ningxia
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| City |
Yinchuan |
| ZIP/Postal code |
750021 |
| Country |
China |
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| Platforms (1) |
| GPL26012 |
Illumina NovaSeq 6000 (Bos taurus) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA997979 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE238109_All_gene_fpkm.list.txt.gz |
578.9 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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