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| Status |
Public on Dec 31, 2010 |
| Title |
Contribution of CgPDR1-regulated genes in enhanced virulence of azole-resistant Candida glabrata (part 2) |
| Platform organism |
Nakaseomyces glabratus CBS 138 |
| Sample organism |
Nakaseomyces glabratus |
| Experiment type |
Expression profiling by array
|
| Summary |
In this study, we aimed to determine genome-wide changes in gene expression driven by seven individual CgPDR1 hyperactive alleles as compared to wild-type allele to identify i) the CgPdr1p target genes differentially expressed in presence of CgPDR1 hyperactive alleles and ii) potential virulence factor(s) regulated by CgPDR1 hyperactive alleles. Microarray experiments revealed a high number of genes (ranging from 80 to 400 genes) differentially regulated by individual CgPDR1 hyperactive alleles.
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| Overall design |
Gene expression was measured in 2 C. glabrata clinical isolates (DSY717 and DSY2317). DSY2317 is azole-susceptible and contains a wild-type CgPDR1 allele. DSY717 is azole-resistant and contains a CgPDR1 allele with the gain-of-function mutation L1081F.
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| Contributor(s) |
Sanglard D, Ferrari S |
| Citation(s) |
21408004 |
| |
| Submission date |
Aug 26, 2010 |
| Last update date |
Jul 22, 2016 |
| Contact name |
Dominique Sanglard |
| E-mail(s) |
Dominique.Sanglard@chuv.ch
|
| URL |
http://www.chuv.ch/imul
|
| Organization name |
University of Lausanne and University Hospital Center
|
| Street address |
Rue Bugnon 48
|
| City |
Lausanne |
| ZIP/Postal code |
1011 |
| Country |
Switzerland |
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| Platforms (1) |
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| Samples (6)
|
| GSM587471 |
strain DSY2317; wt PDR1 gene DSY2317_rep1 |
| GSM587472 |
strain DSY2317; wt PDR1 gene DSY2317_rep2 |
| GSM587473 |
strain DSY2317; wt PDR1 gene DSY2317_rep3 |
| GSM587474 |
strain DSY717; PDR1 with substitution L1081F DSY717_rep1 |
| GSM587475 |
strain DSY717; PDR1 with substitution L1081F DSY717_rep2 |
| GSM587476 |
strain DSY717; PDR1 with substitution L1081F DSY717_rep3 |
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| This SubSeries is part of SuperSeries: |
| GSE23829 |
Contribution of CgPDR1-regulated genes in enhanced virulence of azole-resistant Candida glabrata |
|
| Relations |
| BioProject |
PRJNA133153 |
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