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| Status |
Public on Oct 19, 2010 |
| Title |
Kinetic gene expression profiles of chicken macrophage HD11 cells in response to endotoxin from Salmonella typhimurium-798 |
| Organism |
Gallus gallus |
| Experiment type |
Expression profiling by array
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| Summary |
HD11 cells were stimulated with 1 ug/ml endotoxin from ST-798 for 1, 2, 4 and 8 hours
Cells were harvested at 1, 2, 4 and 8 hrs post stimulation and RNA was isolated from these cells and microarray was conducted for these RNA samples using affymetrix genechips
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| Overall design |
The microarray experiment was conducted using three replications. The first two replications each used one experimental unit and one Affymetrix GeneChip for each of the eight combinations of endotoxin dose (treated vs. control) and time (1, 2, 4, or 8 hours after treatment). The third replication was analyzed with four GeneChips for four endotoxin-treated experimental units measured at 1, 2, 4, and 8 hours after treatment, respectively.
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| Contributor(s) |
Ciraci C, Tuggle CK, Nettleton D, Wannemuehler MJ, Lamont SJ |
| Citation(s) |
20929591 |
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| Submission date |
Aug 30, 2010 |
| Last update date |
Mar 22, 2012 |
| Contact name |
Susan J Lamont |
| E-mail(s) |
sjlamont@iastate.edu
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| Organization name |
Iowa State University
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| Department |
Animal Science
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| Street address |
2255 Kildee Hall
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| City |
Ames |
| State/province |
IA |
| ZIP/Postal code |
50011 |
| Country |
USA |
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| Platforms (1) |
| GPL3213 |
[Chicken] Affymetrix Chicken Genome Array |
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| Samples (20)
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| GSM589082 |
1 ug/ml endotoxin stimulation at 1 hours-biological rep 1 |
| GSM589083 |
0 ug/ml endotoxin stimulation at 8 hours-biological rep 1 |
| GSM589084 |
0 ug/ml endotoxin stimulation at 2 hr-biological rep 1 |
| GSM589085 |
0 ug/ml endotoxin stimulation at 1 hour-biological rep 1 |
| GSM589086 |
1 ug/ml endotoxin stimulation at 8 hours-biological rep 1 |
| GSM589087 |
1 ug/ml endotoxin stimulation at 4 hours-biological rep 1 |
| GSM589088 |
0 ug/ml endotoxin stimulation at 4 hours- biological rep 1 |
| GSM589089 |
1 ug/ml endotoxin stimulation at 2 hours-biological rep 1 |
| GSM589090 |
1 ug/ml endotoxin stimulation at 8 hours-biological rep 2 |
| GSM589091 |
1 ug/ml endotoxin stimulation at 4 hours-biological rep 2 |
| GSM589092 |
0 ug/ml endotoxin stimulation at 1 hour-biological rep 2 |
| GSM589093 |
1 ug/ml endotoxin stimulation at 1 hours-biological rep 2 |
| GSM589094 |
0 ug/ml endotoxin stimulation 8 hours-biological rep 2 |
| GSM589095 |
0 ug/ml endotoxin stimulation 2 hours-biological rep 2 |
| GSM589096 |
0 ug/ml endotoxin stimulation 4 hours-biological rep 2 |
| GSM589097 |
1 ug/ml endotoxin stimulation at 2 hours-biological rep 2 |
| GSM589098 |
1 ug/ml endotoxin stimulation at 4 hours-biological rep 3 |
| GSM589099 |
1 ug/ml endotoxin stimulation at 8 hours-biological rep 3 |
| GSM589100 |
1 ug/ml endotoxin stimulation at 1 hours-biological rep 3 |
| GSM589101 |
1 ug/ml endotoxin stimulation at 2 hours-biological rep 3 |
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| Relations |
| BioProject |
PRJNA130613 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE23881_RAW.tar |
66.9 Mb |
(http)(custom) |
TAR (of CEL) |
| GSE23881_fold-change_q-values.txt.gz |
1.5 Mb |
(ftp)(http) |
TXT |
| Processed data included within Sample table |
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