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| Status |
Public on Aug 01, 2023 |
| Title |
Janus nanoparticles targeting extracellular polymeric substance achieve flexible elimination of drug-resistant biofilms |
| Organism |
Staphylococcus aureus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Safe and efficient antibacterial materials are urgently needed to combat drug-resistant bacteria and biofilm-associated infections. The rational design of nanoparticles for flexible elimination of biofilms by alternative strategies remains challenging. Herein, we propose the fabrication of Janus-structured nanoparticles targeting extracellular polymeric substance to achieve dispersion or near-infrared (NIR) light-activated photothermal elimination of drug-resistant biofilms, respectively. Asymmetrical Janus-structured dextran-bismuth selenide (Dex-BSe) nanoparticles are fabricated by a facile strategy to exploit synergistic effects of both components. The biocompatible dextran domain with the maximum exposure endows the Janus nanoparticles with biofilm penetration, targeting, and dispersion functions. Interestingly, Janus Dex-BSe nanoparticles realize enhanced dispersal of biofilms over time compared with dextran nanoparticles while the underlying molecular mechanisms are further revealed by RNA-sequencing transcriptomics analysis. Alternatively, taking advantage of the preferential accumulation of nanoparticles at infection sites, the self-propelled active motion induced by the unique Janus structure enhances the photothermal killing effect under NIR light irradiation, thereby eradicating the biofilm. Given these favorable features, the antibiofilm activity of Janus Dex-BSe against methicillin-resistant Staphylococcus aureus (MRSA) was first validated in vitro. More importantly, the flexible application of Janus Dex-BSe nanoparticles for biofilm removal or NIR-triggered eradication in vivo was demonstrated by MRSA-infected mouse excisional wound model and abscess model, respectively. The currently developed Janus nanoplatform holds great promise for the efficient elimination of drug-resistant biofilms in diverse antibacterial scenarios.
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| Overall design |
To investigate the function of BS, Dex and DexBS in the regualtion of S. aureus biofilms, We evaluate the gene expression of S. aureus biofilms after treated by Ctrl, BS, Dex,DexBS through RNA-Seq. We then performed gene expression profiling analysis using data obtained from RNA-Seq of different treated S. aureus.
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| Contributor(s) |
Liu Z, Guo K |
| Citation(s) |
37612285 |
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| Submission date |
Jul 27, 2023 |
| Last update date |
Sep 14, 2023 |
| Contact name |
Liu Zhiwen |
| E-mail(s) |
zhiwenliu1996@gmail.com
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| Phone |
15531997435
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| Organization name |
Beijing University of Chemical Technolgoy
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| Street address |
NO.15 Of North Three-ring East Road,Chao Yang District
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| City |
Beijing |
| ZIP/Postal code |
100029 |
| Country |
China |
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| Platforms (1) |
| GPL29442 |
BGISEQ-500 (Staphylococcus aureus) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA999229 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE239411_processed_data.csv.gz |
34.5 Kb |
(ftp)(http) |
CSV |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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