|
| Status |
Public on Jul 28, 2023 |
| Title |
Transcriptional response of USA300 MRSA to coculture with EGM139 Desemzia incerta |
| Organism |
Staphylococcus aureus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We wished to examine the effect that coculture with Desemzia incerta would have on the S. aureus transcriptional profile. We have previously shown D. incerta to have antimicrobial activity against S. aureus. We used a transwell coculture system to separate the two bacterial cultures. Transcriptional profiling revealed downregulated of genes in the arc operon, the pur operon, the mnh family proteins, and cap family proteins and upregulations in the arg operon and the ahp opeorn. Subsequent biological follow-up revealed decrease in S. aureus biofiom formation after D. incerta co-culture.
|
| |
|
| Overall design |
EGM139 D. incerta and USA300 Rosenbach MRSA were cocultured in a Corning 12 well transwell system as described in Wei et al. S. aureus RNA was extracted from the lower well using the ZymoResearch DirecZol RNA-Purification Kit. Sequencing was performed by SeqCenter. 4 replicates of S. aureus cocultured with D. incerta or TSB control were used
|
| |
|
| Contributor(s) |
Wei MH, Knight SA, Grice EA |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| P30 AR069589 |
Penn Skin Biology and Diseases Resource-based Center |
UNIVERSITY OF PENNSYLVANIA |
Elizabeth Anne Grice |
| R00 AR060873 |
The Microbiome of Impaired Wound Healing |
UNIVERSITY OF PENNSYLVANIA |
Elizabeth Anne Grice |
| R01 AR079856 |
Microbial regulation of the keratinocyte AHR |
UNIVERSITY OF PENNSYLVANIA |
Elizabeth Anne Grice |
| R01 NR015639 |
Decoding the microbial burden in diabetic foot ulcers |
UNIVERSITY OF PENNSYLVANIA |
Elizabeth Anne Grice |
|
| Submission date |
Jul 28, 2023 |
| Last update date |
Jul 29, 2023 |
| Contact name |
Monica H Wei |
| Organization name |
University of Pennsylvania
|
| Department |
Department of Dermatology
|
| Lab |
Grice Lab
|
| Street address |
421 Curie Blvd, BRB II/III 1046
|
| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19104 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL30817 |
NextSeq 2000 (Staphylococcus aureus) |
|
| Samples (8)
|
| GSM7666470 |
MRSA monoculture in TSB, replicate 4 |
| GSM7666471 |
MRSA coculture with D. incerta, replicate 1 |
| GSM7666472 |
MRSA coculture with D. incerta, replicate 2 |
| GSM7666473 |
MRSA coculture with D. incerta, replicate 3 |
| GSM7666474 |
MRSA coculture with D. incerta, replicate 4 |
|
| Relations |
| BioProject |
PRJNA999623 |
Less...
More...