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| Status |
Public on Sep 15, 2010 |
| Title |
Stress response regulators identified through genome-wide transcriptome analysis of the (p)ppGpp-dependent response in Rhizobium etli |
| Organism |
Rhizobium etli CFN 42 |
| Experiment type |
Expression profiling by genome tiling array
|
| Summary |
The impact on gene expression by the alarmone (p)ppGpp during growth and non-growth was determined by comparing the transcriptome of R. etli CFN42 wild type and rel mutant. This allowed us to better understand the pleiotropic stress phenotype of the rel mutant as well as identifying specific (p)ppGpp-dependent stress regulators.
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| Overall design |
In order to study expression, R. etli CFN42 wild type and rel mutant was grown at 30˚C in acid minimal salts medium supplied with 10 mM NH4Cl and 10 mM succinate while monitoring the optical density (OD) of the culture. Samples were taken at OD600 = 0.3, 0.7 and 6 hours after reaching the maximum OD, representing early/late exponential and stationary phase, respectively.
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| Contributor(s) |
Vercruysse M, Fauvart M, Michiels J, Cloots L, Engelen K |
| Citation(s) |
21324192 |
| Submission date |
Sep 03, 2010 |
| Last update date |
Aug 19, 2019 |
| Contact name |
Kristof Engelen |
| Organization name |
KULeuven
|
| Lab |
CMPG
|
| Street address |
Kasteelpark Arenberg 20
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| City |
Leuven |
| ZIP/Postal code |
3001 |
| Country |
Belgium |
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| Platforms (1) |
| GPL9409 |
Rhizobium etli CFN 42 385K 071203_ReCFN42JM_Tiling |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA130387 |
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