|
| Status |
Public on Nov 15, 2023 |
| Title |
Single-cell CRISPR screens in vivo map T cell fate regulomes in cancer [Ikzf1 ATAC-Seq] |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
The gene regulatory network (GRN) underlying CTL differentiation and whether Tex responses can be functionally reinvigorated are incompletely understood. Here, we systematically mapped causal GRNs via single-cell CRISPR (scCRISPR) screens in vivo and discovered checkpoints for CTL differentiation.
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| |
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| Overall design |
We used ATAC seq to compare chromatin accessibility profiles of Ikzf1 KO and Spike Tpex and Tex OT-I T cells from the dual-color transfer system at day 7 post adoptive transfer to B16-OVA tumor
|
| Web link |
https://www.nature.com/articles/s41586-023-06733-x
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| |
|
| Contributor(s) |
Zhou P, Shi H, Huang H, Chi H |
| Citation(s) |
37968405 |
| |
| Submission date |
Aug 01, 2023 |
| Last update date |
Nov 17, 2023 |
| Contact name |
Hongbo Chi |
| E-mail(s) |
hongbo.chi@stjude.org
|
| Organization name |
St Jude Children's Research Hospital
|
| Department |
Immunology
|
| Street address |
262 Danny Thomas Place
|
| City |
Memphis |
| State/province |
TN |
| ZIP/Postal code |
38105 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
|
| Samples (20)
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| This SubSeries is part of SuperSeries: |
| GSE216800 |
Single-cell CRISPR screens in vivo map T cell fate regulomes in cancer |
|
| Relations |
| BioProject |
PRJNA1000987 |
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