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Series GSE23986 Query DataSets for GSE23986
Status Public on Aug 25, 2017
Title Dengue virus type-3 isolated from a fatal case with visceral complications induces potent inflammatory responses associated with apoptosis in human monocyte-derived dendritic cells
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Dengue virus (DENV) infection is one of the most serious public health problems worldwide. A recent dengue outbreak in Paraguay (2007-2009) presented unusual manifestations such as hepatitis, encephalitis, pulmonary as well as cardiac disorders associated with 50% of deaths caused by dengue in the country. Despite the knowledge on inflammatory responses observed during the course of disease, the role of innate immune cells in the control of virus replication influencing clinical outcome is poorly defined. Using two clinical isolates of the virus, a non-fatal case of classical DF (DENV3/290) and a fatal case of DF with visceral complications (DENV3/5532), we sought to determine the profile of dengue infection in human dendritic cell, a major innate immune cell population. Compared to classical DENV3/290, the strain DENV3/5532 displayed higher replicative ability in mdDCs. In addition, DENV3/5532 was found to induce elevated production of pro-inflammatory cytokines associated with higher rates of programmed cell death. The observed phenotype was due to viral replication in mdDCs and TNF appeared to display a protective effect on virus-induced mdDCs apoptosis. These results suggest that the fatal case DENV3/5532 isolate modulates dendritic cell survival as well as inflammatory mediator’s synthesis.
Overall design In this study we analyzed the mRNA pool of 10 cultures. Each culture was infected with MOI of 5.0, and the cells were extracted at the times points of 06, 12, 24, and 48 hours after infection.
As infection control, the same 10 cultures were mock-infected. This mRNA pool is used to hibridizated 8 chips, with no replicates.
Contributor(s) Silveira GF, Meyer F, Delfraro A, Mosimann AP, Coluch N, Vasquez C, Probst CM, Báfica A, Bordignon J, Santos CN
Citation(s) 21450836
Submission date Sep 07, 2010
Last update date Jul 26, 2018
Contact name Guilherme Ferreira Silveira
Organization name Instituto Carlos Chagas
Street address Prof Algacyr Munhoz Máder
City Curitiba
ZIP/Postal code 81350-010
Country Brazil
Platforms (1)
GPL6244 [HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [transcript (gene) version]
Samples (8)
GSM590832 5532 - 06h
GSM590833 5532 - 12h
GSM590834 5532 - 24h
BioProject PRJNA130483

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Supplementary file Size Download File type/resource
GSE23986_RAW.tar 34.6 Mb (http)(custom) TAR (of CEL, CHP)
Processed data included within Sample table
Processed data provided as supplementary file

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