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| Status |
Public on Sep 07, 2012 |
| Title |
Comparative genome hybridization profiles of six strains of Lactococcus lactis |
| Organism |
Lactococcus lactis |
| Experiment type |
Genome variation profiling by array
|
| Summary |
The intra sub-species diversity of six strains of Lactococcus lactis subsp. lactis was investigated at the genomic level and in terms of phenotypic and transcriptomic profiles in UF-cheese model. Six strains were isolated from various sources, but all are exhibiting a dairy phenotype. Our results showed that, the six strains exhibited small phenotypic differences since similar behaviour in terms of growth was obtained during cheese ripening while only different acidification capability was detected. Even if all strains displayed high genomic similarities, sharing a high core genome of almost two thousands genes, the expression of this core genome directly in the cheese matrix revealed major strain-specific differences. This strains with the same dairy origin.
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| Overall design |
Strains were cultured on M17. At least three independent cultures of the six strains were performed. Genomic DNA was extracted from cells grown overnight on M17 and radiolabelled cDNA were prepared and hybridized on nylon arrays. 1948 amplicons specific of Lactococcus lactis IL1403 genes were spotted twice on the array. 3 independent repetitions were performed.
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| Contributor(s) |
Tan-a-ram P, Cardoso T, Daveran-Mingot M, Kanchanatawee S, Loubiere P, Girbal L, Cocaign-Bousquet M |
| Citation(s) |
21131529 |
| |
| Submission date |
Sep 07, 2010 |
| Last update date |
Sep 07, 2012 |
| Contact name |
Muriel Cocaign-Bousquet |
| E-mail(s) |
cocaign@insa-toulouse.fr
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| URL |
http://www.insa-toulouse.fr/fr/recherche/labo/lisbp.html
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| Organization name |
Laboratoire d'Ingénierie des Systèmes Biologiques et des Procédés
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| Department |
UMR5504 INSA/CNRS - UMR792 INSA/INRA
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| Street address |
135 avenue de Rangueil
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| City |
Toulouse |
| ZIP/Postal code |
31077 |
| Country |
France |
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| Platforms (1) |
| GPL6414 |
INSA Lactococcus lactis IL1403 (lot de PCR n°2) |
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| Samples (21)
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| GSM590795 |
L. lactis UCMA5713 CGH M17 replicate 1 |
| GSM590796 |
L. lactis UCMA5713 CGH M17 replicate 2 |
| GSM590797 |
L. lactis UCMA5713 CGH M17 replicate 3 |
| GSM590798 |
L. lactis LD55 CGH M17 replicate 1 |
| GSM590799 |
L. lactis LD55 CGH M17 replicate 2 |
| GSM590800 |
L. lactis LD55 CGH M17 replicate 3 |
| GSM590801 |
L. lactis LD61 CGH M17 replicate 1 |
| GSM590802 |
L. lactis LD61 CGH M17 replicate 2 |
| GSM590803 |
L. lactis LD61 CGH M17 replicate 3 |
| GSM590804 |
L. lactis LL08 CGH M17 replicate 1 |
| GSM590805 |
L. lactis LL08 CGH M17 replicate 2 |
| GSM590806 |
L. lactis LL08 CGH M17 replicate 3 |
| GSM590807 |
L. lactis LL52 CGH M17 replicate 1 |
| GSM590808 |
L. lactis LL52 CGH M17 replicate 2 |
| GSM590809 |
L. lactis LL52 CGH M17 replicate 3 |
| GSM590810 |
L. lactis S86 CGH M17 replicate 1 |
| GSM590811 |
L. lactis S86 CGH M17 replicate 2 |
| GSM590812 |
L. lactis S86 CGH M17 replicate 3 |
| GSM590813 |
L. lactis IL1403 CGH M17 replicate 1 |
| GSM590814 |
L. lactis IL1403 CGH M17 replicate 2 |
| GSM590815 |
L. lactis IL1403 CGH M17 replicate 3 |
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| This SubSeries is part of SuperSeries: |
| GSE24015 |
Genomic and transcriptomic analyses of Lactococcus lactis strains |
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| Relations |
| BioProject |
PRJNA133195 |
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