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Series GSE24048 Query DataSets for GSE24048
Status Public on Sep 09, 2010
Title Expression data from field droughted rice plants
Organism Oryza sativa
Experiment type Expression profiling by array
Summary This study aims to provide a transcriptomics dataset for field grown rice plants subjected to mild drought concentrating on the two parents of a mapping population, Bala and Azucena. Plants were grown in 1.2 m2 plots under flooded conditions in Wuhan, China being sown on 2nd June 2007. Starting at 59 days after sowing, drought was imposed by withholding water, while a set of control plots had continued flooding conditions. The drought was imposed for 24 days during which time a small amount of water was added on 3 occasions to raise soil moisture to 30% by volume. After 24 days the second youngest fully expanded leaf was taken and gene expression analysis performed.
We used microarrays to detail the global programme of gene expression underlying drought in rice plants with the aim of using the data to identify candidate genes for drought avoidance QTLs detected within the a rice mapping population.
 
Overall design Two rice cultivars, Bala and Azucena, were grown in 1.2 m2 plots under flooded conditions in Wuhan, China being sown on 2nd June 2007. Starting at 59 days after sowing, drought was imposed by withholding water, while a set of control plots had continued flooding conditions. At 2 pm on the 83rd day after sowing (after 24 days of drought) the second youngest fully expanded leaf was taken off three plants in two plots per block, the leaves had the top and bottom 4 cm removed and the central portion of the leaf was placed in a bag and then into liquid N2. For the controls there was only one plot of the genotypes per block. There was one bag for each block and three replicate blocks. A total of 6 droughted leaf samples (3 Bala and 3 Azucena) and six control leaf samples (3 Bala and 3 Azucena) were collected for RNA extraction and hybridization on Affymetrix microarrays.
 
Contributor(s) Price AH, Jones HG, Norton GJ, Huang S, Xiong L
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Submission date Sep 09, 2010
Last update date Dec 27, 2017
Contact name Gareth Norton
E-mail(s) g.norton@abdn.ac.uk
Organization name University of Aberdeen
Street address Crucikshank building
City Aberdeen
ZIP/Postal code AB24 3UU
Country United Kingdom
 
Platforms (1)
GPL2025 [Rice] Affymetrix Rice Genome Array
Samples (12)
GSM591761 Control Azucena leaf biologial rep 1
GSM591762 Control Azucena leaf biologial rep 2
GSM591763 Control Azucena leaf biologial rep 3
Relations
BioProject PRJNA130249

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE24048_RAW.tar 53.7 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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