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Series GSE240510 Query DataSets for GSE240510
Status Public on Feb 27, 2024
Title The AsiDNA™ decoy mimicking DSBs protects the normal tissue from radiation toxicity through a DNA-PK/p53/p21-dependent G1/S arrest.
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary AsiDNA, a cholesterol-coupled oligonucleotide mimicking double-stranded DNA breaks, was developed to sensitize tumour cells to radio- and chemotherapy. This drug acts as a decoy hijacking the DNA damage response. Previous studies have demonstrated that standalone AsiDNA administration is well tolerated with no additional adverse effects when combined with chemo- and/or radiotherapy. This lack of normal tissue complication encouraged further examination into the role of AsiDNA in normal cells. The delayed development of pulmonary fibrosis after FLASH-RT compared to CONV-RT might be a known phenomenon but the complexity behind this specific tissue response is completely unidentified. Radiation has been shown to impact various cell types presented in the lung, resulting in changes in cellular activity and numerous pathway activations. Current unpublished data suggest a clear variety between the CONV and FLASH-RT gene signatures examined using single cell RNA sequencing. As the combined treatment of AsiDNA with CONV-RT resulted in a delay in radiation induced lung fibrosis compared to CONV-RT standalone, it is to be questioned if the signature of AsiDNA CONV-RT is similar to FLASH-RT or CONV-RT. To characterize the similarity in cellular changes and expression signatures in lungs 5 months post CONV-RT, FLASH-RT, AsiDNA CONV-RT or non-irradiated (Control) treatment. Single cell RNA sequencing of irradiated lungs revealed a closer resemblance between CONV AsiDNA and FLASH-RT in profibrotic gene signatures within the fibroblast and macrophages population, in comparison to CONV-RT standalone. Collectively, this information indicates that the activity of AsiDNA and FLASH-RT could draw upon identical mechanism interference to result in the protective capacities within the normal tissue.
Overall design Single-cell suspensions were prepared from 5 month C57BL/6J mice and single cell RNA-seq libraries were generated using 3' V3 or 3'V3 NextGem chemistry kit on Chromium Single cell controller (10x Genomics). This dataset includes 3 samples: 1 sample 5 months 13 Gy post-CONV irradiation; 1 sample 5 months 13 Gy post-CONV irradiation with AsiDNA drug treatment; 1 sample 5 months 13 Gy post-FLASH irradiation.
Contributor(s) Sesink A, Dubail M, Soulier J, Fouillade C, Girard PM
Citation(s) 38476631
Submission date Aug 09, 2023
Last update date Mar 20, 2024
Contact name Anouk Sesink
Organization name Institut Curie
Street address Rue Henri Becquerel
City Orsay
ZIP/Postal code 91401
Country France
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (3)
GSM7699256 20220307_M41_WT_13Gy_CONV_5M_2644
GSM7699257 20220307_M41_WT_13Gy_CONV_AsiDNA_5M_2620
GSM7699258 20220307_M41_WT_13Gy_FLASH_5M_2626
BioProject PRJNA1003948

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE240510_Hyp_IR_100vC_EdgeR_output.txt.gz 339.5 Kb (ftp)(http) TXT
GSE240510_Hyp_IR_2180vC_EdgeR_output.txt.gz 333.1 Kb (ftp)(http) TXT
GSE240510_Hyp_IR_500vC_EdgeR_output.txt.gz 332.4 Kb (ftp)(http) TXT
GSE240510_Hyp_IR_counts_s2.txt.gz 662.5 Kb (ftp)(http) TXT
GSE240510_RAW.tar 41.3 Mb (http)(custom) TAR (of CSV)
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Processed data provided as supplementary file

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