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| Status |
Public on Jan 25, 2024 |
| Title |
miRNA-Seq analysis differentially expressed miRNAs in intestinal tissues of ASMVT rat |
| Organism |
Rattus |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
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| Summary |
Acute superior mesenteric vein thrombosis (ASMVT) decreases the expression of junction-associated proteins and the number of intestinal epithelial cells, which leads to intestinal epithelial barrier disruption. Thermoprotein deposition has also recently been identified as an important cause of mucosal barrier defects. However, the role and mechanism of thermoprotein deposition in asmvt is not fully understood. Differentially expressed microRNAs (miRNAs) in the intestinal tissues of ASMVT mice were detected by transcriptome sequencing (RNA-Seq). To identify miRNAs involved in intestinal barrier disruption, we performed RNA-Seq analysis of intestinal tissues from ASMVT mice . Sixty miRNAs were abnormally expressed in normal intestinal tissues compared with ASMVT-induced intestinal tissues.
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| Overall design |
6-8 weeks male Sprague Dawley rats (weight, 300–350 g) were supplied by the Hunan SJA Laboratory Animal Co., Ltd and housed individually in a temperature-controlled barrier facility with light-dark (12:12) cycles at 22 ℃ with 50% humidity, and received ad libitum food and water. The animal experiments have been approved by the Animal Ethics Committee of Kunming University of Science and Technology.The ASMVT rat model was established according to the method described in a previous study [25]. The rats were fasted for 12 hours before the operation and were given free access to water. In the lower abdomen of the rat, the rat was anesthetized with 5% ketamine 2 ml + 0.05% atropine 1 ml + 2.5% promethazine 2 ml at 5% ketamine 2 ml + 0.05% atropine 1 ml + 2.5% promethazine 2 ml at 2 ml/kg, and a midline incision of approximately 2 cm in length was made in the upper abdomen. The position of the cecum was determined after entering the abdominal cavity. The small intestine was pulled 5 cm away from the ileocecal region and the mesentery was exposed. The main branches of the mesenteric vein and the ends of the arch vein are ligated with 7-0 nylon thread. During mesenteric vein clamping, approximately 15-20 ml/kg of saline is injected intraperitoneally intermittently to prevent transient hypovolemia after loosening of the arterial clamp. Mesenteric vein dilatation and intestinal congestion were dark red, indicating successful modeling. After closing the abdomen, 25-30 m/kg of saline (containing gentamicin 320,000 U/L) was added into the abdominal cavity to prevent abdominal surgical infection. After surgery, the rats were placed back into the feeding cage for observation, one rat per cage, and were allowed to eat and drink freely. Rats were randomly divided into the following groups: (1) Sham group: after surgical exposure of the superior mesenteric vein; (2) ASMVT group;Rats were executed by spinal dislocation method under deep anesthesia. The affected intestine was cut down the middle and the intestinal contents were washed with normal saline. Small intestinal tissues were stored at -80°C until use.
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| Citation missing |
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| BioProject |
PRJNA1002815 |
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| Submission date |
Aug 10, 2023 |
| Last update date |
Jan 25, 2024 |
| Contact name |
kunmei Gong |
| E-mail(s) |
kunhuagongkunmei@outlook.com
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| Organization name |
The first People's Hospital of Yunnan Province
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| Street address |
Jinbi Road 157
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| City |
Yunnnan province |
| ZIP/Postal code |
650034 |
| Country |
China |
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| Platforms (1) |
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| Samples (6)
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| GSM7699390 |
adult male small intestine,Rat ASMVT surgical model,rep1 |
| GSM7699391 |
adult male small intestine,Rat ASMVT surgical model,rep2 |
| GSM7699392 |
adult male small intestine,Rat ASMVT surgical model,rep3 |
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| Supplementary file |
Size |
Download |
File type/resource |
| GSE240523_Table5_All_Expressed_miRNA.txt.gz |
117.6 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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