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| Status |
Public on Jan 25, 2024 |
| Title |
5-Hydroxymethylcytosine signals in serum are a predictor of chemoresistance in high-grade serous ovarian cancer |
| Organism |
Homo sapiens |
| Experiment type |
Methylation profiling by high throughput sequencing
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| Summary |
The genome-wide profiling of 5-hydroxymethylcytosine (5hmC) on circulating cell-free DNA (cfDNA) has revealed promising biomarkers for a variety of diseases. The purpose of our study is to investigate if 5hmC profiles from serum cfDNA are novel predictive biomarkers for the development of chemoresistance in high-grade serous ovarian cancer (HGSOC). We hypothesized that 5-hmC profiles associate with the development of chemoresistance and elucidate pathways that may drive chemoresistance in HGSOC. We developed a novel multivariate model based on clinico-pathologic data and a cfDNA-derived 5hmC-annotated gene that can predict response to platinum-based chemotherapy in intermediate-sensitive HGSOC. These results merit further investigation of our model as a predictive model.
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| Overall design |
Patients diagnosed with HGSOC and with known platinum sensitivity status (Across all cohorts: sensitive n= 40, intermediate n= 34, resistant n= 37, and recurrent n= 20) were selected for this study. Nano-hmC-Seal was performed on cfDNA isolated from archived serum samples and differential 5hmC annotations were identified using DESeq2 to establish a model predictive of chemoresistance.
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| Web link |
https://doi.org/10.1016/j.ygyno.2024.01.001
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| |
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| Contributor(s) |
Weigert M, Cui X, West-Szymanski D, Zhang W, He C, Lengyel ER |
| Citation(s) |
38262243 |
| |
| Submission date |
Aug 10, 2023 |
| Last update date |
Jan 26, 2024 |
| Contact name |
Xiaolong Cui |
| E-mail(s) |
xiaolong.cui@northwestern.edu
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| Organization name |
Northwestern University
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| Department |
Department of Preventive Medicine
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| Street address |
680 N Lake Shore Drive
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| City |
Chicago |
| State/province |
IL |
| ZIP/Postal code |
60611 |
| Country |
USA |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (131)
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| Relations |
| BioProject |
PRJNA1004198 |
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