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| Status |
Public on Aug 24, 2023 |
| Title |
Identification of a c-type heme oxygenase and its function during acclimation of cyanobacteria to nitrogen fluctuations |
| Organism |
Synechocystis sp. PCC 6803 |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The mechanisms of acclimating to a nitrogen-fluctuating environment are necessary for the survival of aquatic cyanobacteria in their natural habitats, but our understanding is still far from complete. Here, the synthesis of phycobiliprotein is confirmed to be much earlier than that of photosystem components during recovery from nitrogen chlorosis and an unknown protein Ssr1698 is discovered to be involved in this synthetic process. The unknown protein is further identified as a c-type heme oxygenase (cHO) in tetrapyrrole biosynthetic pathway and catalyzes the opening of heme ring to form biliverdin IXα, which is required for phycobilin production and ensuing phycobiliprotein synthesis. In addition, the cHO-dependent phycobiliprotein is found to be vital for the growth of cyanobacterial cells during chlorosis and regreening through its nitrogen-storage and light-harvesting functions, respectively. Collectively, the cHO expressed preferentially during recovery from nitrogen chlorosis is identified in photosynthetic organisms and the dual function of this enzyme-dependent phycobiliprotein is proposed to be an important mechanism for acclimation of aquatic cyanobacteria to a nitrogen-fluctuating environment.
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| Overall design |
A comparative transcriptomic analysis was conducted after the nitrate was added to chlorotic cells for 4 h or not
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| Contributor(s) |
Ma W, Li D, Wei L, Ran Z, Du Z, Miao G, Zheng M, Luo L, Pang X |
| Citation(s) |
37714932 |
| Submission date |
Aug 21, 2023 |
| Last update date |
Sep 27, 2023 |
| Contact name |
Weimin Ma |
| E-mail(s) |
wma@shnu.edu.cn
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| Organization name |
Shanghai Normal University
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| Department |
College of Life Sciences
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| Lab |
Weimin Ma
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| Street address |
No.100 Guilin Rd
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| City |
Shanghai |
| ZIP/Postal code |
200234 |
| Country |
China |
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| Platforms (1) |
| GPL31267 |
Illumina NovaSeq 6000 (Synechocystis sp. PCC 6803) |
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| Samples (6)
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| GSM7727549 |
Synechocystis, continuous low nitrogen, rep1 |
| GSM7727550 |
Synechocystis, continuous low nitrogen, rep2 |
| GSM7727551 |
Synechocystis, continuous low nitrogen, rep3 |
| GSM7727552 |
Synechocystis, low-to-high nitrogen 4 h, rep1 |
| GSM7727553 |
Synechocystis, low-to-high nitrogen 4 h, rep2 |
| GSM7727554 |
Synechocystis, low-to-high nitrogen 4 h, rep3 |
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| Relations |
| BioProject |
PRJNA1007730 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE241350_Low-to-high_nitrogen_for_4h_vs_Continuous_low_nitrogen.txt.gz |
312.9 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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