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| Status |
Public on Sep 04, 2023 |
| Title |
aristaless-like homeobox-3 is wound induced in muscle and stem cells and is required for planarian head regeneration |
| Organism |
Schmidtea mediterranea |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
To investigate the role of alx-3 in activating wound-induced transciptional programs during anterior regneneraiton, control(RNAi) and alx-3(RNAi) planarians were amputated below the pharynx and were allowed to regenerate for 0.5 hours post-amputation (HPA), 6HPA, 24HPA, 48HPA and 72HPA. Intact animals were are also included.Tissue from tail fragments and intact animals was collected at each time point for RNA extraction and cDNA synthesis.
Gene expression profiling analysis was performed using data obtained from RNA-seq of planarian tissues at 5 timepoints after regeneraiton and in uninjured animals .
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| Overall design |
control(RNAi) and alx-3(RNAi) planarians were amputated below the pharynx and were allowed to regenerate for 0.5 hours post-amputation (HPA), 6HPA, 24HPA, 48HPA and 72HPA. Intact animals were are also included.Tissue from tail fragments and intact animals was collected at each time point for RNA extraction and cDNA synthesis.
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| Contributor(s) |
Scidmore TJ, Pearson BJ |
| Citation(s) |
37681295 |
| |
| Submission date |
Aug 23, 2023 |
| Last update date |
Oct 25, 2023 |
| Contact name |
Bret J Pearson |
| E-mail(s) |
pearsobr@ohsu.edu
|
| Organization name |
Oregon Health and Science University
|
| Department |
Pediatrics
|
| Lab |
Pearson
|
| Street address |
3181 SW Sam Jackson Park Rd., BRB Room 305
|
| City |
Portland |
| State/province |
OR |
| ZIP/Postal code |
97239 |
| Country |
USA |
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| Platforms (1) |
| GPL29967 |
Illumina NovaSeq 6000 (Schmidtea mediterranea) |
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| Samples (36)
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| Relations |
| BioProject |
PRJNA1008560 |
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