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| Status |
Public on Sep 17, 2010 |
| Title |
Chromatin enrichment of TRIM24, estrogen receptor and H3K4me2 in estrogen-treated and -untreated MCF7 cells |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
TRIM24 PHD-Bromo domains exhibit preferential binding to unmethylated H3K4 and acetylated H3K27. TRIM24 is a co-activator of estrogen receptor (ER). The results suggest that specific ER-binding sites are depleted of H3K4me2 with estrogen treatment. TRIM24 binds these sites preferentially and facilitates ER-regulated gene expression, cell survival and proliferation.
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| Overall design |
ChIP performed on MCF7 cells +/- estrogen with antibodies against ER, TRIM24 and H3K4me2. ChIP assays of ER, co-activator TRIM24 and H3K4me2 were performed with two concentrations of antibody, without and 6h with estrogen treatment of MCF7 cells. Antibody-enriched samples were sequenced two times, and compared to an IgG negative control and Input. Enriched DNA sequenced by Illumina Solexa.
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| Contributor(s) |
Barton MC, Aronow BJ, Akdemir KC, Jameson C, Keddache M, Yiu TT |
| Citation(s) |
21164480 |
| Submission date |
Sep 16, 2010 |
| Last update date |
May 15, 2019 |
| Contact name |
Bruce J Aronow |
| E-mail(s) |
bruce.aronow@chmcc.org
|
| Phone |
513-636-4865
|
| Organization name |
Cincinnati Children's Hospital Medical Center
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| Street address |
|
| City |
Cincinnati |
| State/province |
OH |
| ZIP/Postal code |
45229 |
| Country |
USA |
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| Platforms (1) |
| GPL9115 |
Illumina Genome Analyzer II (Homo sapiens) |
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| Samples (7)
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| Relations |
| SRA |
SRP003566 |
| BioProject |
PRJNA130083 |
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