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| Status |
Public on Sep 14, 2023 |
| Title |
Metabolic engineering of oleaginous yeast in lipogenic phase enhances production of nervonic acid |
| Organism |
Rhodotorula toruloides |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Insufficient biosynthesis efficiency can be a major obstacle to engineer oleaginous yeasts to overproduce very long-chain fatty acids (VLCFAs) during the lipogenic phase. Taking nervonic acid (NA, C24:1) as an example, we overcame the bottleneck to overproduce NA in engineered Rhodosporidium toruloides by improving the biosynthesis of VLCFAs during the lipogenic phase. First, evaluating the catalytic preferences of three plant-derived ketoacyl-CoA synthases rationally guided reconstructing efficient NA biosynthetic pathway in R. toruloides. More importantly, a genome-wide transcriptional analysis endowed clues to strengthen the fatty acid elongation (FAE) module and identify/use lipogenic phase-activated promoter, collectively addressing the stagnation of NA accumulation during the lipogenic phase. The best-designed strain exhibited a high NA content (major component in total fatty acid [TFA], 46.3%) and produced a titer of 44.2 g/L in a 5 L bioreactor. The strategy developed here provides an engineering framework to establish the microbial process of producing valuable VLCFAs in oleaginous yeasts.
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| Overall design |
To understand the underlying mechanism of the weakening productivity of the VLCFA biosynthesis during the lipogenic phase, we first conducted a genome-wide comparative transcriptomic analysis using Rhodosporidium toruloides strains grown at 48h and 120h, respectively.
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| Contributor(s) |
Liu F, Ma X, Su E |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Aug 24, 2023 |
| Last update date |
Sep 14, 2023 |
| Contact name |
Xiaoqiang Ma |
| E-mail(s) |
xq_ma@sjtu.edu.cn
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| Organization name |
Shanghai Jiaotong University
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| Street address |
800 DONG CHUAN ROAD
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| City |
Shanghai |
| ZIP/Postal code |
200240 |
| Country |
China |
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| Platforms (1) |
| GPL32811 |
Illumina NovaSeq 6000 (Rhodotorula toruloides) |
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| Samples (18)
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| GSM7734213 |
WT_120h, rep1 |
| GSM7734214 |
WT_120h, rep2 |
| GSM7734215 |
WT_120h, rep3 |
| GSM7734216 |
NA04_48h, rep1 |
| GSM7734217 |
NA04_48h, rep2 |
| GSM7734218 |
NA04_48h, rep3 |
| GSM7734219 |
NA04_120h, rep1 |
| GSM7734220 |
NA04_120h, rep2 |
| GSM7734221 |
NA04_120h, rep3 |
| GSM7734222 |
NA14_48h, rep1 |
| GSM7734223 |
NA14_48h, rep2 |
| GSM7734224 |
NA14_48h, rep3 |
| GSM7734225 |
NA14_120h, rep1 |
| GSM7734226 |
NA14_120h, rep2 |
| GSM7734227 |
NA14_120h, rep3 |
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| Relations |
| BioProject |
PRJNA1009202 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE241709_gene_FPKM.txt.gz |
1.4 Mb |
(ftp)(http) |
TXT |
| GSE241709_raw_count.txt.gz |
824.1 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
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