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Series GSE24216 Query DataSets for GSE24216
Status Public on Feb 08, 2011
Title Amplification and overexpression of vinculin are associated with increased tumor cell proliferation and progression in advanced prostate cancer
Organism Homo sapiens
Experiment type Genome variation profiling by genome tiling array
Summary We analyzed the 10q22 amplification in advanced prostate cancer and subjected the genes located in the common amplified region to an RNAi screen. We found vinculin as the most promising candidate gene and analyzed its protein expression on more than 400 prostate cancers by using the tissue micrarray (TMA) technology. We discovered a strong correlation between amplification of the vinculin gene and increased protein expression. Further, vinculin protein expression was strongly associated with increased tumor cell proliferation.
Overall design We isolated DNA from four 10q22 amplified cell lines (PC-3, MFM-223, SK-BR-3, ME-180). Genomic DNA was digested with the restriction enzymes RSA I / ALU I and then labeled with Cy5 using a Klenow-based commercial kit (Invitrogen). Each sample was hybridized with a pooled normal (46,XX) reference (Promega) to Agilent 244k CGH arrays. This experiment allowed us to define the common amplified region of the 10q22 amplification.
Contributor(s) Ruiz C, Holz DR, Oeggerli M, Schneider S, Gonzales IM, Kiefer JM, Zellweger T, Bachmann A, Koivisto PA, Helin HJ, Mousses S, Barrett MT, Azorsa DO, Bubendorf L
Citation(s) 21294127
Submission date Sep 20, 2010
Last update date Oct 26, 2016
Contact name Christian Ruiz
Phone +41 61 265 3152
Fax +41 61 265 2966
Organization name University Hospital Basel
Department Institute for Pathology
Street address Schoenbeinstrasse 40
City Basel
ZIP/Postal code 4031
Country Switzerland
Platforms (1)
GPL4091 Agilent-014693 Human Genome CGH Microarray 244A (Feature number version)
Samples (4)
GSM595615 PC3
GSM595616 MFM223
GSM595617 SKBR3
BioProject PRJNA133021

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SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE24216_RAW.tar 271.1 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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