 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Feb 08, 2011 |
| Title |
Amplification and overexpression of vinculin are associated with increased tumor cell proliferation and progression in advanced prostate cancer |
| Organism |
Homo sapiens |
| Experiment type |
Genome variation profiling by genome tiling array
|
| Summary |
We analyzed the 10q22 amplification in advanced prostate cancer and subjected the genes located in the common amplified region to an RNAi screen. We found vinculin as the most promising candidate gene and analyzed its protein expression on more than 400 prostate cancers by using the tissue micrarray (TMA) technology. We discovered a strong correlation between amplification of the vinculin gene and increased protein expression. Further, vinculin protein expression was strongly associated with increased tumor cell proliferation.
|
| |
|
| Overall design |
We isolated DNA from four 10q22 amplified cell lines (PC-3, MFM-223, SK-BR-3, ME-180). Genomic DNA was digested with the restriction enzymes RSA I / ALU I and then labeled with Cy5 using a Klenow-based commercial kit (Invitrogen). Each sample was hybridized with a pooled normal (46,XX) reference (Promega) to Agilent 244k CGH arrays. This experiment allowed us to define the common amplified region of the 10q22 amplification.
|
| |
|
| Contributor(s) |
Ruiz C, Holz DR, Oeggerli M, Schneider S, Gonzales IM, Kiefer JM, Zellweger T, Bachmann A, Koivisto PA, Helin HJ, Mousses S, Barrett MT, Azorsa DO, Bubendorf L |
| Citation(s) |
21294127 |
| |
| Submission date |
Sep 20, 2010 |
| Last update date |
Oct 26, 2016 |
| Contact name |
Christian Ruiz |
| E-mail(s) |
christian.ruiz@unibas.ch
|
| Phone |
+41 61 265 3152
|
| Fax |
+41 61 265 2966
|
| Organization name |
University Hospital Basel
|
| Department |
Institute for Pathology
|
| Street address |
Schoenbeinstrasse 40
|
| City |
Basel |
| ZIP/Postal code |
4031 |
| Country |
Switzerland |
| |
|
| Platforms (1) |
| GPL4091 |
Agilent-014693 Human Genome CGH Microarray 244A (Feature number version) |
|
| Samples (4)
|
|
| Relations |
| BioProject |
PRJNA133021 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE24216_RAW.tar |
271.1 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
|
|
|
|
 |
Less...
More...